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MBP/MBL ELISA Kit

MBP/MBL Reaktivität: Human Colorimetric Sandwich ELISA 16-1000 pg/mL Cell Lysate, Plasma, Serum
Produktnummer ABIN1380039
  • Target Alle MBP/MBL ELISA Kits anzeigen
    MBP/MBL (Mannan Binding Protein/Mannan Binding Lectin (MBP/MBL))
    Reaktivität
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    16-1000 pg/mL
    Untere Nachweisgrenze
    16 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    The OmniKine? Human MBL ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human MBL concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human MBL while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non- specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
    Marke
    OmniKine™
    Proben
    Cell Lysate, Serum, Plasma
    Analytische Methode
    Quantitative
    Spezifität
    The Human MBL ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human MBL proteins.
    Kreuzreaktivität (Details)
    The Human MBL ELISA is capable of recognizing both recombinant and naturally produced Human MBL proteins. The antigens listed below were tested at 50 ng/mL and did not exhibit significant cross reactivity or interference. Murine: MBL-1, MBL-2
    Produktmerkmale
    The Human MBL ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human MBL proteins within the range of 16-1000 pg/mL.
    Bestandteile
    • Microstrips Coated w / Capture Antibody: 12 x 8-Well Microstrips
    • Protein Standard: Lyophilized (100 ng), Red container
    • Biotinylated Detection Antibody: Lyophilized, Yellow container
    • 400x Streptavidin-HRP: 30 μL, Blue container
    • Wash Buffer (10x): 50 mL, Clear containter
    • Assay Diluent: 50 mL, Clear container
    • Ready-to-Use Substrate: 12 mL, Brown container
    • Stop Solution: 12 mL, Clear container
    • Adhesive Plate Sealers: 4 Sheets
    • Technical Manual 1 Manual
    Benötigtes Material
    The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:
    Microplate reader able to measure absorbance at 450 nm (with correction wavelength set to 540 nm or 570 nm)
    Micropipettes with capability of measuring volumes ranging from 1 μl to 1 mL
    Deionized or sterile water
    Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
    Graph paper or computer software capable of generating or displaying logarithmic functions
    Absorbent paper or vacuum aspirator
    Test tubes or microfuge tubes capable of storing ≥1 mL
    Bench
    top centrifuge (optional)
    Bench
    top vortex (optional)
    Orbital shaker (optional)
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  • Plattentyp
    Pre-coated
    Protokoll
    This particular immunoassay utilizes the quantitative technique of a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a sandwich format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human MBL cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and sandwiching of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’- Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
    Aufbereitung der Proben

    If samples are to be used within 24 hours, aliquot and store at 4 °C. If samples are to be used over a long period of time, aliquot and store between -20 °C and -80 °C, depending on the duration of storage.
    Note: Samples containing a visible precipitate or pellet must be clarified prior to use in the assay.
    Caution: Avoid repeated freeze/thaw cycles to prevent loss of biological activity of proteins in experimental samples.

    • Cell Lysate and Supernatants:
      Remove large cell components via centrifugation and perform the assay. Cell lysates and supernatants require a dilution using Assay Diluent. A serial dilution may be performed to determine a suitable dilution factor for the sample. For future use of the sample, follow the sample storage guidelines stated above.
    • Serum:
      Allow samples to clot in a serum separator tube (SST) for 30 minutes. After sufficient clotting, centrifuge at 1000 x g for 15 minutes and remove serum from SST in preparation for the assay. Serum samples require at least a 1:50 dilution using Assay Diluent. For future use of the sample, follow the storage guidelines above.
    • Plasma:
      Use heparin, citrate or EDTA as an anticoagulant to gather plasma from original biological sample. After collection of the plasma, centrifuge for 15 minutes at 1000 x g. This step must be performed within 30 minutes of plasma collection. Plasma samples require at least a 1:50 dilution using Assay Diluent. Afterwards, perform the assay or for future use of the sample, follow the storage guidelines stated above.

    Testdurchführung

    Note: If possible, all incubation steps should be performed on an orbital shaker to equilibrate solutions when added to the microplate wells. Also, all provided solutions should be at ambient temperature prior to use.
    Note: Avoid adding solutions into wells at an angle, always keep pipette tip perpendicular to plate bottom.

    Reconstitution of Provided Materials:

      1. Reconstitute the Biotin-Conjugated Detection Antibody in 67 µL of ddH₂O for a concentration of 180 µg/ml.
      2. Reconstitute the Protein Standard in 100 µL of ddH₂O for a concentration of 340 ng/ml.
      3. Dilute the 50 mL of 10x Wash Buffer in 450 mL of ddH2O for 500 mL of 1x Wash Buffer.
    Addition of Known Standard and Unknown Sample to Immunoassay:
      The OmniKine™ Human CD163 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CD163 proteins

    Ergebnisberechnung

    Generation of Standard Curve and Interpretation of Data
    1. Average the duplicate or triplicate readings for each standard, control and sample and subtract the average zero standard optical density.
    2. Generate a standard curve by using Microsoft Excel or other computer software capable of establishing a 4- Parameter Logistic (4-PL) curve fit. If using Excel or an alternative graphing tool, plot the average optical density values in absorbance units (y-axis) against the known standard concentrations in pg/ml (x-axis). Note: Only use the values in which a noticeable gradient can be established. Afterwards, generate a best fit curve or trend-line through the plotted points via regression analysis.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Vorsichtsmaßnahmen
    Reagents provided in this kit may be harmful if ingested, inhaled or absorbed through the skin. Please carefully review the MSDS for each reagent before conducting the experiment.
    Stop Solution contains 2 N Sulfuric Acid (H2SO4) and is an extremely corrosive agent. Please wear proper eye, hand and face protection when handling this material. When the experiment is finished, be sure to rinse the plate with copious amounts of running water to dilute the Stop Solution prior to disposing the plate.
    Handhabung
    This ELISA kit is intended for research purposes only, NOT diagnostic or clinical procedures of any kind.
    Materials included in this kit should NOT be used past the expiration date on the kit label.
    Reagents or substrates included in this kit should NOT be mixed or substituted with reagents or substrates from any other kits.
    Variations in pipetting technique, washing technique, operator laboratory technique, kit age, incubation time or temperature may cause differences in binding affinity of the materials provided.
    The assay is designed to eliminate interference and background by other cellular macromolecules or factors present within any biological samples. However, the possibility of background noise cannot be fully excluded until all factors have been tested using the assay kit.

    Reagents provided in this kit may be harmful if ingested, inhaled or absorbed through the skin. Please carefully review the MSDS for each reagent before conducting the experiment.
    Stop Solution contains 2 N Sulfuric Acid (H2SO4) and is an extremely corrosive agent. Please wear proper eye, hand and face protection when handling this material. When the experiment is finished, be sure to rinse the plate with copious amounts of running water to dilute the Stop Solution prior to disposing the plate.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Note: If used frequently, reagents may be stored at 4 °C.
    • Unopened Kits: Store at 4 °C for 6 months.
    • Microstrips Coated w/ Capture Antibody, 400x Streptavidin-HRP Wash Buffer (10x), Assay Diluent Ready-to-Use Substrate, Stop Solution: 6 Months at 4 °C
    • Protein Standard, Biotinylated Detection Antibody: Lyophilized: 6 Months (if Reconstituted: 1 Month) at 4 °C
  • Yang, Seeney, Wolter, Anders, McCormack, Tunnicliffe, Rabnott, Shaw, Dent, Kim, Zimmerman, Fong: "Mannose-binding lectin gene polymorphism predicts hospital admissions for COPD infections." in: Genes and immunity, Vol. 4, Issue 4, pp. 269-74, (2003) (PubMed).

  • Target Alle MBP/MBL ELISA Kits anzeigen
    MBP/MBL (Mannan Binding Protein/Mannan Binding Lectin (MBP/MBL))
    Andere Bezeichnung
    MBL (MBP/MBL Produkte)
    Synonyme
    COLEC1 ELISA Kit, HSMBPC ELISA Kit, MBL ELISA Kit, MBL2D ELISA Kit, MBP ELISA Kit, MBP-C ELISA Kit, MBP1 ELISA Kit, MBPD ELISA Kit, pMBP-27 ELISA Kit, MBL1 ELISA Kit, cMBl ELISA Kit, collectin ELISA Kit, L-MBP ELISA Kit, MBL-C ELISA Kit, RaRF p28A ELISA Kit, mbl ELISA Kit, mannose binding lectin 2 ELISA Kit, mannose-binding lectin (protein C) 2 ELISA Kit, mannose-binding protein C-like ELISA Kit, MBL2 ELISA Kit, Mbl2 ELISA Kit, LOC100911854 ELISA Kit
    Hintergrund
    MBL is a calcium-dependent lectin involved in innate immune defense. It binds mannose, fructose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. MPL also binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. MBL may bind DNA. MBL consists of an oligomeric complex of 3 or more homotrimers. This lectin interacts with MASP1 and MASP2. Interactions with MEP1A and MEP1B may inhibit their catalytic activities. Genetic variations in MBL2 influence susceptibility to hepatitis B virus (HBV) infection. There is an association between low levels of MBL2 and a defect of opsonization which results in susceptibility to frequent and chronic infections. Source: Entrez Gene, Swiss-Prot
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