Salmonella IgG ELISA Kit

Produktnummer ABIN1326893

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human Salmonella IgG.

Kurzübersicht für Salmonella IgG ELISA Kit (ABIN1326893)

Target: Salmonella IgG

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Applikation: ELISA

Proben: Serum

Produktdetails

Verwendungszweck: Diluted patient serum is added to wells coated with purified antigen. IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample.

Analytische Methode: Qualitative

Anwendungsinformationen

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Antigendetails

Target: Salmonella IgG

Substanzklasse: Antibody

Hintergrund: ISalmonella typhii is the causative agent of typhoid fever a contagious infection of the intestines that affects the whole body. In developing countries, typhoid often occurs in epidemics. Most people in the United States get typhoid as a result of visiting another country where the food or water supply has been contaminated. Symptoms usually start 1 to 3 weeks after exposure to the bacteria. Symptoms include: high fever, headache, sore throat, vomiting, diarrhea, skin rash and weakness. The symptoms may take 2 weeks or more to go away. Typhoid is spread when a person drinks or eats food and water contaminated by human waste (stool or urine) containingi Salmonella typhii bacteria.A person who no longer has symptoms may still transmit the bacteria as a carrier. Testing for immunoglobulin G ( IgG), IgA, and IgM antilipopolysaccharide (LPS) of iSalmonellai typhi antibodies by enzyme-linked immunosorbent assay (iELISAi) showed that the levels of all three classes of immunoglobulin anti-LPS of S. typhi were higher in typhoid patients than in healthy or febrile nontyphoidal groups. The iELISAi assay was much more sensitive and specific than any combination of the Widal test, and hence it could be a useful tool for the serologic diagnosis of typhoidal fever with a single blood sample.