Cortisol ELISA Kit

Produktnummer ABIN1326818

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Schaf Cortisol.

Kurzübersicht für Cortisol ELISA Kit (ABIN1326818)

Target: Cortisol

Reaktivität: Schaf

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Applikation: ELISA

Produktdetails Cortisol ELISA Kit

Verwendungszweck: The Cortisol is a solid phase competitive ELISA. The samples and Cortisol enzyme conjugate are added to the wells coated with anti-Cortisol monoclonal antibody. Cortisol in the patient's sample competes with a Cortisol enzyme conjugate for binding sites. Unbound Cortisol and Cortisol enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of Cortisol in the samples. A standard curve is prepared relating color intensity to the concentration of the Cortisol.

Analytische Methode: Quantitative

Anwendungsinformationen

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu Cortisol

Target: Cortisol

Substanzklasse: Hormone

Hintergrund: Cortisol (hydrocortisone, compound F) is the most potent glucocorticoid synthesized from cholesterol. Cortisol is found in the blood either as free Cortisol, or bound to corticosteroid-binding globulin (CBG). Cortisol production has an ACTH-dependent circadian rhythm with peak levels in the early morning and a nadir at night. The factors controlling this circadian rhythm are not completely defined. Serum levels are highest in the early morning and decrease throughout the day. In the metabolic aspect, Cortisol promotes gluconeogenesis, liver glycogen deposition, and the reduction of glucose utilization. Immunologically, Cortisol functions as an important anti inflammatory, and plays a role in hypersensitivity, immunosuppression, and disease resistance. This kit can detect low level of cortisol in sheep serum or plasma (less than 0.1 ngml).