Fecal Rota-Adeno Duo Antigen Rapid Test Kit

Produktnummer ABIN1305186

Kurzübersicht für Fecal Rota-Adeno Duo Antigen Rapid Test Kit (ABIN1305186)

Target: Rota-Adeno Duo Antigen

Reaktivität: Virus

Applikation: Rapid Test (rapid)

Proben: Fecal

Analytische Methode: Qualitative

Produktdetails

Verwendungszweck: This is a two-in-one test including a rotavirus antigen test strip and an adenovirus antigen test strip that are back-to-back positioned in one test tube. The rotavirus and adenovirus Duo antigen rapid test strips employ two group paired monoclonal antibodies for either rotavirus antigen or adenovirus antigen. Dye-conjugated monoclonal antibodies against antigen VP6 of group A of rotavirus, and solid-phase specific rotavirus are one of the antibody pair. The antibodies specifically against human genus adenovirus antigens and a solid-phase coated monoclonal antibody specifically against adenovirus hexon antigens form another paired antibody set. In this test the specimen is first treated with an extraction solution to extract rotavirus and/or adenovirus antigens from the feces. Following extraction, the only step required is to screw the rotavirus and adenovirus Duo test strip tube into the sample collection device. As the sample extraction flows through the chamber and reach the test strips, the colored particles migrate. In the case of a positive result the specific antibodies present on the membrane will capture the colored particles. Different colored lines will be visible, depending upon the virus content of the sample. These lines, after 5 minutes of incubation at room temperature, are used to interpret the result

Kreuzreaktivität (Details): Cross reactivity has been evaluated and found to be negative compared to positive specimens of Cryptosporidium parvum and Giardia lamblia.

Bestandteile: 1. Rotavirus and Adenovirus Duo test strip 30
2. Fecal sample collection device with single use pipette 30
3. Green pipet for liquid stool sample collection 30
4. Instruction for use 1

Benötigtes Material: 1. Disposable pipette for watery sample collection
2. Positive Control

Anwendungsinformationen

Kommentare:

Single pouched.

Testdauer: 0.5 h

Protokoll: This rotavirus and adenovirus Duo antigen test kit is intended for the direct qualitative detection of the presence of rotavirus and/or adenovirus antigens in patient fecal samples. The test might be used as an aid for detecting patients with acute gastroenteritis infected with rotavirus and/or adenovirus.

Probennahme: Stool samples must be taken as soon as the symptoms appear. Viral particles decrease in number after one week, making the diagnosis more difficult. It is strongly recommended to test the freshly collected sample as soon as possible. The samples can be stored in the refrigerator for 24 hours. For longer storage they must be kept frozen at -20 °C. In this case, the sample should be totally thawed, and brought to room temperature and homogenized before testing. For each solid specimen: Unscrew the sampling lid and keep the sampling tube in a vertical position to prevent the loss of any extraction solution. Insert and twist the tip of the sampling lid into the stool specimen at two or more different sites. Collect fecal sample that is stuck to the surface of the sampling lid. Do not intentionally collect any separate and large pieces of fecal sample into the tube. Replace the sampling lid into the tube and secure tightly. For each liquid specimen: Unscrew the sampling lid and keep the sampling tube in a vertical position to prevent the loss of any extraction solution. Using the green pipet to collect three drops of the liquid stool sample and add to the sampling tube. Replace the sampling lid into the tube and secure tightly.

Testdurchführung:

1. Bring foil pouch sealed test strips and collected specimens to room temperature (8 - 30 C). 2. Mix the sampling tube vigorously to ensure a good liquid suspension. 3. Position the sampling tube upper side down in vertical and let it sediment for about 1 minute. 4. Remove the test strip from the sealed foil pouch. 5. Screw the test strip in a vertical position into the sampling tube by breaking into the bottom seal of the sampling tube. Tighten securely! 6. Allow the solution to flow into the bottom space of the test strip and keep the device in a vertical position. 7. Read test result at 5 minutes and do not interpret test result after 10 minutes.

Ergebnisberechnung:

1. Valid Test: The appearance of control line on the test reading membrane area indicates the test is valid. The control line is located at the upper half of the test membrane area.
   2. Positive: If two red/pink colored bands are visible on either strip within 10 minutes, the test result is positive for the respective Adeno and/or Rota.
   3. Negative: If both rotavirus and adenovirus test lines have no colored band and the control line displays a red/pink colored band, the test result is negative.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: The reagents are for research use only. Source material from which reagents of bovine serum was derived in the contiguous 48 United States. It was obtained only from donor health animals maintained under veterinary supervision and found free of contagious diseases. Wear gloves while performing this test and handle these reagents and patient samples as if they are infectious. Do not get in eyes, on skin, or on clothing. Do not ingest. On contact, flush with copious amounts of water for at least 15 minutes. When the assay procedure is completed, dispose of specimens (biohazard materials) carefully after autoclaving for at least one hour. Alternatively, treat with a 0.5 or 1% solution of sodium hypochlorite for one hour before disposal. Use Good Laboratory Practices.

Lagerung: 4 °C

Antigendetails

Target: Rota-Adeno Duo Antigen

Substanzklasse: Viral Protein

Hintergrund: Rotaviruses are the main cause of acute gastroenteritis and diarrhea, especially in children under the age of two years. Rotaviruses have been identified in almost 40 % of the feces of children with gastroenteritis. Rotavirus is the cause of up to 50 % of the hospitalized cases of diarrhea in infant and young children. If not treated, the infection may result in severe dehydration and disorders of body electrolyte balance. Therefore, it can be mortal in risk populations such as children, the elderly or immunosuppressed individuals. Rotavirus is transmitted by oral-fecal contact with an incubation period of 1-3 days. Characteristic symptoms include vomiting, hydrodiarrhoea for between 3 and 8 days, high temperature and stomach pains. A large amount of rotavirus particles is shed during infection. Adenoviruses are one of the main causes of acute gastroenteritis and diarrhea, especially in children under the age of two years. Adenoviruses have been identified in almost 12 % of the feces of children with gastroenteritis. It was reported that adenovirus is the second leading cause of the hospitalized cases of diarrhea in infant and young children. If not treated, the infection may result in severe dehydration and disorders of body electrolyte balance. Therefore, it can be mortal in risk populations such as children, the elderly or immunosuppressed individuals. Adenovirus is transmitted by oral-fecal contact, but can result from the inhalation of aerosols as well. Its incubation period lasts 5 to 8 days. Characteristic symptoms include vomiting, hydrodiarrhoea, high temperature and stomach pains. There are 41 known human adenoviruses differentiated primarily by serological and DNA analysis. Morphologically, the viruses are non-enveloped icosahedral structure with their diameter of about 80 nm. Diagnosis of gastroenteritis with rotavirus and/or adenovirus infection can be established based on the detection of the virus particles by electron microscopy or the virus antigen by specific immunoassay methods.