TOLLIP ELISA Kit

Produktnummer ABIN1161909

Das Human TOLLIP ELISA Kit (ABIN1161909) ist ein Colorimetric ELISA Kit zur Detektion von Human TOLLIP.

Kurzübersicht für TOLLIP ELISA Kit (ABIN1161909)

Target: TOLLIP (Toll Interacting Protein (TOLLIP))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2-2000 pg/mL

Applikation: ELISA

Produktdetails TOLLIP ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Verwendungszweck: This immunoassay kit allows for the in vitro quantitative determination of human Toll-interacting protein, concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Analytische Methode: Quantitative

Spezifität: This assay recognizes recombinant and natural human. No significant cross-reactivity or interference was observed.

Bestandteile: Reagent (Quantity): Assay plate (1), Standard (2), Sample Diluent (1x20ml), Assay Diluent A (1x10ml), Assay Diluent B (1x10ml), Detection Reagent A (1x120µl), Detection Reagent B (1x120µl), Wash Buffer(25 x concentrate) (1x30ml), Substrate (1x10ml), Stop Solution (1x10ml)

Anwendungsinformationen

Kommentare:

Gene Name: TOLLIP

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: The microtiter plate provided in this kit has been pre-coated with an antibody specific to . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Details zu TOLLIP

Target: TOLLIP (Toll Interacting Protein (TOLLIP))

Andere Bezeichnung: Toll-interacting protein

Hintergrund: Synonyms: Homo sapiens,Human,Toll-interacting protein,TOLLIP
Subunit: Oligomerizes. Binds to TLR2 and the TLR4-MD2 complex via its C-terminus. Exists as complex with IRAK1 in unstimulated cells. Upon IL-1 signaling, Tollip binds to the activated IL-1 receptor complex containing IL-1RI, IL-1RacP and the adapter protein MyD88, where it interacts with the TIR domain of IL-1RacP. MyD88 then triggers IRAK1 autophosphorylation, which in turn leads to the dissociation of IRAK1 from Tollip and IL-1RAcP. Interacts with TOM1L2.
Function: Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity.

UniProt: Q9H0E2