PPT1 ELISA Kit

Produktnummer ABIN1146897

Das Human PPT1 ELISA Kit (ABIN1146897) ist ein Colorimetric ELISA Kit zur Detektion von Human PPT1.

Kurzübersicht für PPT1 ELISA Kit (ABIN1146897)

Target: PPT1 (Palmitoyl-Protein Thioesterase 1 (PPT1))

Reaktivität: Human

Nachweismethode: Colorimetric

Detektionsbereich: 0.62-40.0 ng/mL

Applikation: ELISA

Produktdetails PPT1 ELISA Kit

Untere Nachweisgrenze: 0.62 ng/mL

Produktmerkmale: The ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PPT-1. During the reaction, PPT-1 in the sample or standard competes with a fixed amount of biotin-labeled PPT-1 for sites on a pre-coated Monoclonal antibody specific to PPT-1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PPT-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Bestandteile: Reagent (Quantity): Assay plate (1), Standard (2), Sample Diluent (1x20ml), Assay Diluent A (1x10ml), Assay Diluent B (1x10ml), Detection Reagent A (1x120µl), Detection Reagent B (1x120µl), Wash Buffer(25 x concentrate) (1x30ml), Substrate (1x10ml), Stop Solution (1x10ml)

Anwendungsinformationen

Kommentare:

Gene Name: PPT1

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Details zu PPT1

Target: PPT1 (Palmitoyl-Protein Thioesterase 1 (PPT1))

Andere Bezeichnung: Palmitoyl-protein thioesterase 1

Hintergrund: Synonyms: Homo sapiens,Human,Palmitoyl-protein hydrolase 1,Palmitoyl-protein thioesterase 1,PPT,PPT1,PPT-1

Pathways: SARS-CoV-2 Protein Interaktom