CPS1 ELISA Kit (Carbamoyl-Phosphate Synthase 1, Mitochondrial)

Details for Product CPS1 ELISA Kit No. ABIN1125223, Anbieter: Anmelden zum Anzeigen
Antigen
  • CPSASE1
  • cps
  • 4732433M03Rik
  • CPS
  • D1Ucla3
  • Cps1
  • carbamoyl-phosphate synthase 1
  • carbamoyl-phosphate synthase 1 L homeolog
  • carbamoyl-phosphate synthetase 1
  • peptidase M20 domain containing 1
  • CPS1
  • Cps1
  • cps1.L
  • cps1
  • PM20D1
Epitop
Mitochondrial
Reaktivität
Human
Alternativen
Kits mit alternativen Reaktivitäten:
5
2
2
Applikation
ELISA
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
'Independent Validation' Siegel
Antigen CPS1
Chargennummer 7K295N
Validierte Anwendung ELISA
Positivkontrolle Human serum from patients with liver injury
Negativkontrolle Human serum from patients without liver injury
Bewertung

Passed, the CPS1 ELISA kit ABIN1125223 specifically detects the antigen in human serum samples.

Protokoll
  • Freeze human serum samples in aliquots until use. Dilute sera with PBS.
  • Allow all reagents to reach RT and prepare all the reagents, standard solutions and samples as instructed by the manufacturer.
  • Add 100µl of standard, blank or sample per well. Cover the plate. Incubate for 2h at 37°C.
  • Remove the liquid.
  • Add 100µl detection reagent A. Mix gently and cover the plate. Incubate for 1h at 37°C.
  • Aspirate liquid and wash the wells 3x with 400µl wash buffer. Let it sit for 1-2min. Remove liquid Completely at each step.
  • Add 100µl of detection reagent B. Cover the plate. Incubate for 1h at 37°C.
  • Wash well 5x with 400µl wash buffer.
  • Add 90µl substrate solution. Cover the plate. Incubate 15min at 37°C protected from light.
  • Add 50µl stop solution. Gently tap the plate to ensure thorough mixing.
  • Read absorbance at 450nm immediately.
Anmerkungen
  • The purpose of this report is to validate the measurement of human CPS1 in human serum samples, since it has been published that CPS1 is released into serum during liver injury, while it is not detected in healthy human serum (Weerasinghe et al. (2014)).

  • To validate this ELISA, human serum samples previously validated to contain CPS1 by Western blotting were used. The signal in the positive controls samples (patients’ samples that contain high amount of CPS1 which was confirmed by Western blotting) was significantly higher than the blank whereas the signal from the negative control samples (healthy human sera in that CPS1 was not detected by Western blotting) was comparable to the baseline. 1:5, 1:25, or 1:125 diluted serum samples were tested as well.

  • CPS1 was not detected in the sera of healthy human by both western blotting and ELISA method (lane 1 and 2). However, it is highly increased in the sera of patients with acute liver failure and the signals were in a dilution-dependent manner (lane 3 to 11).

  • The standard curve for the assays reported were fitted with a 4-Parameter Logistic model. The calculated concentrations of standards were higher than expected, but it gave a good quality fits (R2=0.999). The results showed no CPS1 in the sera from healthy human, while it is highly increased in the sera from patients with liver injury, as expected. Although most of the samples were in the detection range, the calculated concentrations were highest in the most diluted samples.

Validierungsbilder
ELISA validation image for Carbamoyl-Phosphate Synthase 1, Mitochondrial (CPS1) ELISA Kit (ABIN1125223) A. Standard curve and sample measurements using ABIN1125223. B. The standard curves f...
Nachweismethode Colorimetric
Produktmerkmale Homo sapiens,Human,Carbamoyl-phosphate synthase [ammonia], mitochondrial,Carbamoyl-phosphate synthetase I,CPSase I,CPS1,6.3.4.16
Bestandteile Reagent (Quantity): Assay plate (1), Standard (2), Sample Diluent (1x20ml), Assay Diluent A (1x10ml), Assay Diluent B (1x10ml), Detection Reagent A (1x120µl), Detection Reagent B (1x120µl), Wash Buffer(25 x concentrate) (1x30ml), Substrate (1x10ml), Stop Solution (1x10ml)
Plasmids, Primers & others Plasmide, Primers & weitere CPS1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung CPS1
Hintergrund Synonyms: Carbamoyl-phosphate synthase [ammonia], mitochondrial,Carbamoyl-phosphate synthetase I,CPS1,CPSase I,Homo sapiens,Human
Pathways Response to Growth Hormone Stimulus, Cellular Glucan Metabolic Process
Kommentare

Gene Name: CPS1

Probenmenge 100 μL
Plattentyp Pre-coated
Beschränkungen Nur für Forschungszwecke einsetzbar
Lagerung 4 °C/-20 °C
Informationen zur Lagerung The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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