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CPS1 ELISA Kit

This anti-CPS1 antibody (ABIN1125223) is a antibody detecting CPS1 in ELISA. Suitable for Human.
Produktnummer ABIN1125223

Kurzübersicht für CPS1 ELISA Kit (ABIN1125223)

Target

Alle CPS1 ELISA Kits anzeigen
CPS1 (Carbamoyl-Phosphate Synthase 1, Mitochondrial (CPS1))

Bindungsspezifität

Mitochondrial

Reaktivität

  • 3
  • 1
  • 1
Human

Nachweismethode

Colorimetric

Applikation

ELISA
  • Produktmerkmale

    Homo sapiens,Human,Carbamoyl-phosphate synthase [ammonia], mitochondrial,Carbamoyl-phosphate synthetase I,CPSase I,CPS1,6.3.4.16

    Bestandteile

    Reagent (Quantity): Assay plate (1), Standard (2), Sample Diluent (1x20ml), Assay Diluent A (1x10ml), Assay Diluent B (1x10ml), Detection Reagent A (1x120µl), Detection Reagent B (1x120µl), Wash Buffer(25 x concentrate) (1x30ml), Substrate (1x10ml), Stop Solution (1x10ml)
  • Kommentare

    Gene Name: CPS1

    Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Validierung #103213 (ELISA)
    'Independent Validation' Siegel
    by
    Omary Lab, Department of Molecular & Integrative Physiology, University of Michigan Medical School
    No.
    #103213
    Datum
    11.06.2018
    Antigen
    CPS1
    Chargennummer
    7K295N
    Validierte Anwendung
    ELISA
    Positivkontrolle
    Human serum from patients with liver injury
    Negativkontrolle
    Human serum from patients without liver injury
    Bewertung

    Passed, the CPS1 ELISA kit ABIN1125223 specifically detects the antigen in human serum samples.

    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    Sekundärantikörper
    Full Protocol
    • Freeze human serum samples in aliquots until use. Dilute sera with PBS.
    • Allow all reagents to reach RT and prepare all the reagents, standard solutions and samples as instructed by the manufacturer.
    • Add 100µl of standard, blank or sample per well. Cover the plate. Incubate for 2h at 37°C.
    • Remove the liquid.
    • Add 100µl detection reagent A. Mix gently and cover the plate. Incubate for 1h at 37°C.
    • Aspirate liquid and wash the wells 3x with 400µl wash buffer. Let it sit for 1-2min. Remove liquid Completely at each step.
    • Add 100µl of detection reagent B. Cover the plate. Incubate for 1h at 37°C.
    • Wash well 5x with 400µl wash buffer.
    • Add 90µl substrate solution. Cover the plate. Incubate 15min at 37°C protected from light.
    • Add 50µl stop solution. Gently tap the plate to ensure thorough mixing.
    • Read absorbance at 450nm immediately.
    Anmerkungen
    • The purpose of this report is to validate the measurement of human CPS1 in human serum samples, since it has been published that CPS1 is released into serum during liver injury, while it is not detected in healthy human serum (Weerasinghe et al. (2014)).

    • To validate this ELISA, human serum samples previously validated to contain CPS1 by Western blotting were used. The signal in the positive controls samples (patients’ samples that contain high amount of CPS1 which was confirmed by Western blotting) was significantly higher than the blank whereas the signal from the negative control samples (healthy human sera in that CPS1 was not detected by Western blotting) was comparable to the baseline. 1:5, 1:25, or 1:125 diluted serum samples were tested as well.

    • CPS1 was not detected in the sera of healthy human by both western blotting and ELISA method (lane 1 and 2). However, it is highly increased in the sera of patients with acute liver failure and the signals were in a dilution-dependent manner (lane 3 to 11).

    • The standard curve for the assays reported were fitted with a 4-Parameter Logistic model. The calculated concentrations of standards were higher than expected, but it gave a good quality fits (R2=0.999). The results showed no CPS1 in the sera from healthy human, while it is highly increased in the sera from patients with liver injury, as expected. Although most of the samples were in the detection range, the calculated concentrations were highest in the most diluted samples.

  • Lagerung

    4 °C/-20 °C

    Informationen zur Lagerung

    The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
  • Target Alle CPS1 ELISA Kits anzeigen

    CPS1 (Carbamoyl-Phosphate Synthase 1, Mitochondrial (CPS1))

    Andere Bezeichnung

    CPS1

    Hintergrund

    Synonyms: Carbamoyl-phosphate synthase [ammonia], mitochondrial,Carbamoyl-phosphate synthetase I,CPS1,CPSase I,Homo sapiens,Human

    Pathways

    Response to Growth Hormone Stimulus, Cellular Glucan Metabolic Process
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