ADNP ELISA Kit

Produktnummer ABIN1120362

Dieses Maus ADNP ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus ADNP zu quantifizieren.

Kurzübersicht für ADNP ELISA Kit (ABIN1120362)

Target: ADNP (Activity-Dependent Neuroprotector Homeobox (ADNP))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 0.31-20.0 ng/mL

Applikation: ELISA

Produktdetails ADNP ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Verwendungszweck: This immunoassay kit allows for the in vitro quantitative determination of mouseActivity-dependent neuroprotector homeobox proteinconcentrationsin serum, plasma, tissue homogenates,cell culture supernates, and other biological fluids.

Analytische Methode: Quantitative

Spezifität: This assay recognizes recombinant and natural MouseActivity-dependent neuroprotector homeobox protein. No significant cross-reactivity or interference was observed.

Bestandteile: Reagent (Quantity): Assay plate (1), Standard (2), Sample Diluent (1x20ml), Assay Diluent A (1x10ml), Assay Diluent B (1x10ml), Detection Reagent A (1x60µl), Detection Reagent B (1x120µl), Wash Buffer(25 x concentrate) (1x30ml), Substrate (1x10ml), Stop Solution (1x10ml)

Anwendungsinformationen

Kommentare:

Gene Name: Adnp

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: he ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activity-dependent neuroprotector homeobox protein, during the reaction,Activity-dependent neuroprotector homeobox proteinin the sample or standard competes with a fixed amount of biotin-labeled Activity-dependent neuroprotector homeobox proteinfor sites on a pre-coated Monoclonal antibody specific to Activity-dependent neuroprotector homeobox protein. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentrationof Activity-dependent neuroprotector homeobox proteinin the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Details zu ADNP

Target: ADNP (Activity-Dependent Neuroprotector Homeobox (ADNP))

Andere Bezeichnung: Activity-dependent neuroprotector homeobox protein

Hintergrund: Synonyms: Activity-dependent neuroprotective protein,Activity-dependent neuroprotector homeobox protein,Adnp,Mouse,Mus musculus

Pathways: Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process