AGE ELISA Kit

Produktnummer ABIN1118087

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Diverse Spezies AGE. Es wurde in 26 Publikationen zitiert.

Kurzübersicht für AGE ELISA Kit (ABIN1118087)

Target: AGE (Advanced Glycation End Products (AGE))

Reaktivität: Diverse Spezies

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 98.8 ng/mL - 8000 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails AGE ELISA Kit

Untere Nachweisgrenze: 98.8 ng/mL

Verwendungszweck: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of AGE in Serum,Plasma,Biological Fluids

Analytische Methode: Quantitative

Spezifität:

This assay has high sensitivity and excellent specificity for detection of Advanced Glycation End Product (AGE).
No significant cross-reactivity or interference between Advanced Glycation End Product (AGE) and analogues was observed.

Kreuzreaktivität (Details): No significant cross-reactivity or interference between Advanced Glycation End Product (AGE) and analogues was observed.

Sensitivität: < 38.2 ng/mL

Bestandteile:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• TMB Substrate
• Stop Solution
• Wash Buffer (30X)
• Instruction manual

Benötigtes Material:

• Microplate reader with 450 nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Anwendungsinformationen

Applikationshinweise:

• Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
• The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
• Kits from different batches may be a little different in detection range, sensitivity and color developing time.
• Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
• Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
• There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
• Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
• Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
• Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
• Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.

Kommentare:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Probenmenge: 50 μL

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Testpräzision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Advanced Glycation End Product (AGE) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Advanced Glycation End Product (AGE) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Handhabung: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Lagerung: 4 °C

Informationen zur Lagerung:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Haltbarkeit: 6 months

Referenzen für AGE ELISA Kit (ABIN1118087)

Chou, Wu, Yang, Shen, Yang: "Effect of Advanced Glycation End Products on the Progression of Alzheimer's Disease." in: Journal of Alzheimer's disease : JAD, Vol. 72, Issue 1, pp. 191-197, (2020) (PubMed).

Han, Ma, Zhu, Sun, Liu: "Advanced glycation end products enhance macrophage polarization to the M1 phenotype via the HIF-1α/PDK4 pathway." in: Molecular and cellular endocrinology, Vol. 514, pp. 110878, (2020) (PubMed).

Pawlukianiec, Gryciuk, Mil, Żendzian-Piotrowska, Zalewska, Maciejczyk: "A New Insight into Meloxicam: Assessment of Antioxidant and Anti-Glycating Activity in In Vitro Studies." in: Pharmaceuticals (Basel, Switzerland), Vol. 13, Issue 9, (2020) (PubMed).

Tang, Yu, Wu, Chen, Zeng, Wang, Yang, Mei, Cao, Qin: "Lychee seed extract protects against neuronal injury and improves cognitive function in rats with type II diabetes mellitus with cognitive impairment." in: International journal of molecular medicine, Vol. 41, Issue 1, pp. 251-263, (2018) (PubMed).

Yang, Meng, Chen, Li, Cen, Wen, Zhang, Li: "Inhibition of Methylglyoxal-Induced AGEs/RAGE Expression Contributes to Dermal Protection by N-Acetyl-L-Cysteine." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 41, Issue 2, pp. 742-754, (2017) (PubMed).

Galiniak, Bartosz, Sadowska-Bartosz: "Glutathione is the main endogenous inhibitor of protein glycation." in: General physiology and biophysics, Vol. 36, Issue 2, pp. 175-186, (2017) (PubMed).

Qiu, Tang, Wei: "Berberine exerts renoprotective effects by regulating the AGEs-RAGE signaling pathway in mesangial cells during diabetic nephropathy." in: Molecular and cellular endocrinology, Vol. 443, pp. 89-105, (2017) (PubMed).

Sun, Huang, Xu, Sun, Lv, Wang: "Advanced glycation end products promote VEGF expression and thus choroidal neovascularization via Cyr61-PI3K/AKT signaling pathway." in: Scientific reports, Vol. 7, Issue 1, pp. 14925, (2017) (PubMed).

Winerdal, Winerdal, Wang, Fredholm, Winqvist, Ådén: "Adenosine A1 receptors contribute to immune regulation after neonatal hypoxic ischemic brain injury." in: Purinergic signalling, Vol. 12, Issue 1, pp. 89-101, (2016) (PubMed).

Malmstedt, Frebelius, Lengquist, Jörneskog, Wang, Swedenborg: "The Receptor for Advanced Glycation End Products (Rage) and Its Ligands in Plasma and Infrainguinal Bypass Vein." in: European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery, Vol. 51, Issue 4, pp. 579-86, (2016) (PubMed).

Dong, Zhang, Liu, Zhu, Zhao, Li, Sun, Ding, Hu, Yang, Zhang, Li: "Beneficial effects of urine-derived stem cells on fibrosis and apoptosis of myocardial, glomerular and bladder cells." in: Molecular and cellular endocrinology, Vol. 427, pp. 21-32, (2016) (PubMed).

Kosutova, Mikolka, Kolomaznik, Balentova, Calkovska, Mokra: "Effects of S-Nitroso-N-Acetyl-Penicillamine (SNAP) on Inflammation, Lung Tissue Apoptosis and iNOS Activity in a Rabbit Model of Acute Lung Injury." in: Advances in experimental medicine and biology, Vol. 935, pp. 13-23, (2016) (PubMed).

Sadowska-Bartosz, Galiniak, Skolimowski, Stefaniuk, Bartosz: "Nitroxides prevent protein glycoxidation in vitro." in: Free radical research, Vol. 49, Issue 2, pp. 113-21, (2015) (PubMed).

Xu, Cai, Sun, Liao, Liu, Xiao, Zhu, Liu, Zhang: "Serum advanced glycation end products are associated with insulin resistance in male nondiabetic patients with obstructive sleep apnea." in: Sleep & breathing = Schlaf & Atmung, (2015) (PubMed).

Zhiping, Imam, Ismail, Ismail, Yida, Ideris, Sarega, Mahmud: "Effects of edible bird's nest on hippocampal and cortical neurodegeneration in ovariectomized rats." in: Food & function, Vol. 6, Issue 5, pp. 1701-11, (2015) (PubMed).

Cai, Sun, Liu, Xu, Xiao, Duan, Liu, Zhang: "Relationship between serum levels of endogenous secretory RAGE and blood pressure in male nondiabetic patients with obstructive sleep apnea." in: Journal of human hypertension, Vol. 29, Issue 12, pp. 713-8, (2015) (PubMed).

Sadowska-Bartosz, Stefaniuk, Galiniak, Bartosz: "Glycation of bovine serum albumin by ascorbate in vitro: Possible contribution of the ascorbyl radical?" in: Redox biology, Vol. 6, pp. 93-9, (2015) (PubMed).

Wu, Li, Li, Sui, Yang, Dong, Xie, Sun: "Inhibition of Advanced Glycation Endproduct Formation by Lotus Seedpod Oligomeric Procyanidins through RAGE-MAPK Signaling and NF-κB Activation in High-Fat-Diet Rats." in: Journal of agricultural and food chemistry, Vol. 63, Issue 31, pp. 6989-98, (2015) (PubMed).

Sadowska-Bartosz, Bartosz: "Ascorbic acid and protein glycation in vitro." in: Chemico-biological interactions, Vol. 240, pp. 154-62, (2015) (PubMed).

Li, Zhang, Chen, Zhang, Ma, Xia: "Establishment of a rabbit model to study the influence of advanced glycation end products accumulation on osteoarthritis and the protective effect of pioglitazone." in: Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society, (2015) (PubMed).

Details zu AGE

Target: AGE (Advanced Glycation End Products (AGE))

Andere Bezeichnung: AGE

Substanzklasse: Chemical

Hintergrund: AGEs, Advanced Glycation End Products