ADMA ELISA Kit

Produktnummer ABIN1118084

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Diverse Spezies ADMA. Es wurde in 15 Publikationen zitiert.

Kurzübersicht für ADMA ELISA Kit (ABIN1118084)

Target: ADMA (Asymmetrical Dimethylarginine (ADMA))

Reaktivität: Diverse Spezies

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 12.35 ng/mL - 1000 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Produktdetails ADMA ELISA Kit

Untere Nachweisgrenze: 12.35 ng/mL

Verwendungszweck: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of ADMA in Serum,Plasma,Tissue Homogenate,Cell Lysate,Cell Culture Supernatant,Biological Fluids

Analytische Methode: Quantitative

Spezifität:

This assay has high sensitivity and excellent specificity for detection of Asymmetrical Dimethylarginine (ADMA).
No significant cross-reactivity or interference between Asymmetrical Dimethylarginine (ADMA) and analogues was observed.

Kreuzreaktivität (Details): No significant cross-reactivity or interference between Asymmetrical Dimethylarginine (ADMA) and analogues was observed.

Sensitivität: < 4.99 ng/mL

Bestandteile:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• TMB Substrate
• Stop Solution
• Wash Buffer (30X)
• Instruction manual

Benötigtes Material:

• Microplate reader with 450 nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Anwendungsinformationen

Applikationshinweise:

• Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
• The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
• Kits from different batches may be a little different in detection range, sensitivity and color developing time.
• Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
• Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
• There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
• Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
• Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
• Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
• Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.

Kommentare:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Probenmenge: 50 μL

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Testpräzision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Asymmetrical Dimethylarginine (ADMA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Asymmetrical Dimethylarginine (ADMA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Handhabung: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Lagerung: 4 °C

Informationen zur Lagerung:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Haltbarkeit: 6 months

Referenzen für ADMA ELISA Kit (ABIN1118084)

Hernández, Porta, Bandello, Grauslund, Harding, Aldington, Egan, Frydkjaer-Olsen, García-Arumí, Gibson, Lang, Lattanzio, Massin, Midena, Ponsati, Ribeiro, Scanlon, Cunha-Vaz, Simó: "The Usefulness of Serum Biomarkers in the Early Stages of Diabetic Retinopathy: Results of the EUROCONDOR Clinical Trial." in: Journal of clinical medicine, Vol. 9, Issue 4, (2020) (PubMed).

Reddy, Dasari, Duscharla, Supriya, Ram, Surekha, Kumar, Ummanni et al.: "Dimethylarginine dimethylaminohydrolase-1 (DDAH1) is frequently upregulated in prostate cancer, and its overexpression conveys tumor growth and angiogenesis by metabolizing asymmetric ..." in: Angiogenesis, Vol. 21, Issue 1, pp. 79-94, (2019) (PubMed).

Kami Reddy, Dasari, Vandavasi, Natani, Supriya, Jadav, Sai Ram, Kumar, Ummanni: "Novel Cellularly Active Inhibitor Regresses DDAH1 Induced Prostate Tumor Growth by Restraining Tumor Angiogenesis through Targeting DDAH1/ADMA/NOS Pathway." in: ACS combinatorial science, Vol. 21, Issue 4, pp. 241-256, (2019) (PubMed).

Shahin, Abdelkader, Safar: "A Novel Role of Irbesartan in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats: Targeting DDAH/ADMA and EGFR/ERK Signaling." in: Scientific reports, Vol. 8, Issue 1, pp. 4280, (2018) (PubMed).

El Assar, Fernández, Sánchez-Ferrer, Angulo, Rodríguez-Mañas: "Multivessel analysis of progressive vascular aging in the rat: Asynchronous vulnerability among vascular territories." in: Mechanisms of ageing and development, Vol. 173, pp. 39-49, (2018) (PubMed).

Xuan, Xu, Tian, Li, Wang, He, Lun: "Dimethylarginine Dimethylaminohydrolase 2 (DDAH 2) Gene Polymorphism, Asymmetric Dimethylarginine (ADMA) Concentrations, and Risk of Coronary Artery Disease: A Case-Control Study." in: Scientific reports, Vol. 6, pp. 33934, (2016) (PubMed).

Xuan, Liu, Wang, Guo, Zhang, He, Lun: "Increased serum concentrations of asymmetric dimethylarginine (ADMA) in patients with early-onset coronary artery disease." in: Clinica chimica acta; international journal of clinical chemistry, Vol. 464, pp. 195-199, (2016) (PubMed).

El Assar, Angulo, Santos-Ruiz, Ruiz de Adana, Pindado, Sánchez-Ferrer, Hernández, Rodríguez-Mañas: "Asymmetric dimethylarginine (ADMA) elevation and arginase up-regulation contribute to endothelial dysfunction related to insulin resistance in rats and morbidly obese humans." in: The Journal of physiology, Vol. 594, Issue 11, pp. 3045-60, (2016) (PubMed).

Zhao, Liu, Ji, Wang, He, Tian, Xu, Lei, Wang: "Effect of 18?-glycyrrhetinic acid on cerebral vasospasm caused by asymmetric dimethylarginine after experimental subarachnoid hemorrhage in rats." in: Neurological research, Vol. 37, Issue 6, pp. 476-83, (2015) (PubMed).

Aydin, Altintas, Cem Mutlu, Bilir, Oran, Tülübaş, Topçu, Tayfur, Küçükyalçin, Kaplan, Gürel: "Asymmetric dimethylarginine contributes to airway nitric oxide deficiency in patients with COPD." in: The clinical respiratory journal, (2015) (PubMed).

Kim, Kim et al.: "Dimethylarginine dimethylaminohydrolase-1 mediates inhibitory effect of interleukin-10 on angiotensin II-induced hypertensive effects in vascular smooth muscle cells of spontaneously hypertensive ..." in: Cytokine, Vol. 77, pp. 203-10, (2015) (PubMed).

Zhao, Zhang, Sun: "Correlation research on ADMA plasma levels and left ventricular function of peritoneal dialysis patients." in: International journal of clinical and experimental medicine, Vol. 7, Issue 11, pp. 4455-60, (2014) (PubMed).

Kim, Kim, Kim: "Effect of CCL5 on dimethylarginine dimethylaminohydrolase-1 production in vascular smooth muscle cells from spontaneously hypertensive rats." in: Cytokine, Vol. 64, Issue 1, pp. 227-33, (2013) (PubMed).

Ali, Mishra, Kumar, Alam, Pandey, Ram, Thinlas, Mohammad, Pasha: "Interactions among vascular-tone modulators contribute to high altitude pulmonary edema and augmented vasoreactivity in highlanders." in: PLoS ONE, Vol. 7, Issue 9, pp. e44049, (2012) (PubMed).

Thorberg, Hall, Akesson, Svensson, Nilsson: "Aminochromans: potent agonists at central dopamine and serotonin receptors." in: Acta pharmaceutica Suecica, Vol. 24, Issue 4, pp. 169-82, (1988) (PubMed).

Details zu ADMA

Target: ADMA (Asymmetrical Dimethylarginine (ADMA))

Andere Bezeichnung: Asymmetrical Dimethylarginine

Hintergrund: N,N-Dimethylarginine, NG,NG-Dimethylarginine dihydrochloride, Asymmetric Dimethylarginine