IgG ELISA Kit
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- Target Alle IgG ELISA Kits anzeigen
- IgG
- Bindungsspezifität
- Heavy & Light Chain
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Reaktivität
- Maus
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 20-1900 ng/mL
- Untere Nachweisgrenze
- 20 ng/mL
- Applikation
- ELISA
- Verwendungszweck
- Rapid quantification of mouse IgG in cell culture supernatant, follow-up of production process
- Marke
- FastELISA®
- Proben
- Cell Culture Supernatant, Ascitic Fluid
- Analytische Methode
- Quantitative
- Spezifität
- The method enables the detection of all IgG (IgG3 quantification requires a specific standard curve).
- Kreuzreaktivität (Details)
- < 1% for Human IgG, < 1.5% for Cow IgG, < 2% for Goat IgG, < 5 % for Swine IgG and < 15 % for Guinea Pig and Rat IgG. The cross reaction with human serum and fetal calf serum is typically below 0.2%.
- Sensitivität
- 6 ng/mL
- Produktmerkmale
- The mouse immunoglobulin quantification kit provides a rapid and easy method (one step ELISA) for the quantitative determination of mouse IgG in cell culture supernatant and ascitic fluid.
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- Discover our top product IgG ELISA Kit
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IgG ELISA Kit
Reaktivität: Kaninchen Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA KitReaktivität: Maus Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA KitReaktivität: Human Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA KitReaktivität: Huhn Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA KitReaktivität: Maus Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA KitReaktivität: Ratte Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA KitReaktivität: Schwein Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA KitReaktivität: Rind (Kuh) Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
Rabbit TrueBlot® Set (with IP beads)WB, IP Reaktivität: Kaninchen
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- Kommentare
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One Step ELISA 30 min total time
- Testdauer
- < 1 h
- Plattentyp
- Pre-coated
- Protokoll
- A polyclonal antibody specific to mouse IgG (H+L) is pre-coated onto microwells. Samples and standards are pipetted into microwells and mouse IgG present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-mouse IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of mouse IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
- Aufbereitung der Reagenzien
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dilution
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- 4 °C
- Haltbarkeit
- 12 months
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- Target Alle IgG ELISA Kits anzeigen
- IgG
- Andere Bezeichnung
- Immunoglobulin G (IgG Produkte)
- Substanzklasse
- Antibody
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