IgG ELISA Kit

Produktnummer ABIN1113188

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus IgG.

Kurzübersicht für IgG ELISA Kit (ABIN1113188)

Target: IgG

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 20-1900 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Ascitic Fluid

Produktdetails IgG ELISA Kit

Untere Nachweisgrenze: 20 ng/mL

Verwendungszweck: Rapid quantification of mouse IgG in cell culture supernatant, follow-up of production process

Marke: FastELISA®

Analytische Methode: Quantitative

Spezifität: The method enables the detection of all IgG (IgG3 quantification requires a specific standard curve).

Kreuzreaktivität (Details): < 1% for Human IgG, < 1.5% for Cow IgG, < 2% for Goat IgG, < 5 % for Swine IgG and < 15 % for Guinea Pig and Rat IgG. The cross reaction with human serum and fetal calf serum is typically below 0.2%.

Sensitivität: 6 ng/mL

Produktmerkmale: The mouse immunoglobulin quantification kit provides a rapid and easy method (one step ELISA) for the quantitative determination of mouse IgG in cell culture supernatant and ascitic fluid.

Anwendungsinformationen

Kommentare:

One Step ELISA 30 min total time

Testdauer: < 1 h

Plattentyp: Pre-coated

Protokoll: A polyclonal antibody specific to mouse IgG (H+L) is pre-coated onto microwells. Samples and standards are pipetted into microwells and mouse IgG present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-mouse IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of mouse IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Aufbereitung der Reagenzien:

dilution

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu IgG

Target: IgG

Andere Bezeichnung: Immunoglobulin G

Substanzklasse: Antibody