CCL20 ELISA Kit

Produktnummer ABIN1112752

Human CCL20 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human CCL20.

Kurzübersicht für CCL20 ELISA Kit (ABIN1112752)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8 pg/mL - 500 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum, Tissue Lysate

Produktdetails CCL20 ELISA Kit

Untere Nachweisgrenze: 7.8 pg/mL

Verwendungszweck: For quantitative detection of MIP-3α in Human serum, plasma, tissue lysates,cell culture supernatants.

Analytische Methode: Quantitative

Sensitivität: < 1.5 pg/mL

Bestandteile: 1. One 96-well plate pre-coated with anti-Human MIP-3alpha antibody 2. Lyophilized Human MIP-3alpha standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Human MIP-3alpha antibody (Concentrated): 130 µl.

Benötigtes Material:

1. 37 °C incubator
2. Microplate reader (wavelength: 450 nm)
3. Precise pipette and disposable pipette tips
4. Automated plate washer
5. ELISA shaker
6. 1.5 mL of Eppendorf tubes
7. Plate cover
8. Absorbent filter papers
9. Plastic or glass container with volume of above 1 L

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MIP-3alpha polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MIP-3alpha polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the MIP-3alpha amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of MIP-3alpha can be calculated.

Plattentyp: Pre-coated

Aufbereitung der Reagenzien:

1. Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37 °C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.

Aufbereitung der Proben:

Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Tissue lysate, body fluids and cell culture supernatants: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with citrate, heparin or EDTA as the anticoagulant. Centrifuge for 15 min at 1000 x g within 30 min of collection. Analyze immediately or aliquot and store frozen at -20 °C. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konservierungsmittel: Sodium azide, Thimerosal (Merthiolate)

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 2-8°C for 6 months, or at -20°C for 12 months.

Haltbarkeit: 12 months

Details zu CCL20

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Andere Bezeichnung: MIP-3alpha

Hintergrund: Macrophage Inflammatory Protein-3 (MIP-3Į), also known as Chemokine (C-C motif) ligand 20 (CCL20) or liver activation regulated chemokine (LARC), is a small cytokine belonging to the CC chemokine family. the CCL20 gene contains 4 exons and maps to chromosome 2q35-q36. It is strongly chemotactic for lymphocytes and weakly attracts neutrophils. CCL20 is expressed in several tissues with highest expression observed in peripheral blood lymphocytes, lymph nodes, liver, appendix, and fetal lung and lower levels in thymus, testis, prostate and gut. CCL20 is implicated in the formation and function of mucosal lymphoid tissues via chemoattraction of lymphocytes and dendritic cells towards the epithelial cells surrounding these tissues. CCL20 elicits its effects on its target cells by binding and activating the chemokine receptor CCR6.

Pathways: Phosphorylierungen bei SARS-CoV-2 Infektion