CXCL10 ELISA Kit

Produktnummer ABIN1112582

Produktdetails CXCL10 ELISA Kit

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2-2000 pg/mL

Untere Nachweisgrenze: 31.2 pg/mL

Applikation: ELISA

Verwendungszweck: For quantitative detection of CXCL10 in mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants.

Proben: Cell Culture Supernatant, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Sensitivität: < 1 pg/mL

Bestandteile: 1. One 96-well plate pre-coated with anti-mouse CXCL10 antibody 2. Lyophilized mouse CXCL10 standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-mouse CXCL10 antibody (Concentrated): 130 µl.

Benötigtes Material: 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L

Anwendungsinformationen

Kommentare:

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CXCL10 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CXCL10 polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the CXCL10 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of CXCL10 can be calculated.

Plattentyp: Pre-coated

Aufbereitung der Reagenzien:

1. Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.

Aufbereitung der Proben:

Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Body fluids, tissue lysate and cell culture supernatants: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C . For cell lysate, add lysis solution before centrifugation.
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 2000 × g for 20 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with heparin or EDTA or citrate as the anticoagulant. Centrifuge for 20 min at 2000 x g within 30 min of collection. Analyze immediately or aliquot and store frozen at -20 °C. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konservierungsmittel: Sodium azide, Thimerosal (Merthiolate)

Details zu CXCL10

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Andere Bezeichnung: CXCL10 / IP-10 (CXCL10 Produkte)

Synonyme: CXCL10 ELISA Kit, C7 ELISA Kit, IFI10 ELISA Kit, INP10 ELISA Kit, IP-10 ELISA Kit, SCYB10 ELISA Kit, crg-2 ELISA Kit, gIP-10 ELISA Kit, mob-1 ELISA Kit, CRG-2 ELISA Kit, IP10 ELISA Kit, Ifi10 ELISA Kit, Scyb10 ELISA Kit, C-X-C motif chemokine ligand 10 ELISA Kit, C-X-C motif chemokine 10 ELISA Kit, chemokine (C-X-C motif) ligand 10 ELISA Kit, CXCL10 ELISA Kit, cxl10 ELISA Kit, Cxcl10 ELISA Kit

Hintergrund: C-X-C motif chemokine 10 (CXCL10) also known as Interferon gamma-induced protein 10 (IP-10) or small-inducible cytokine B10 is an 8.7 kDa protein that in humans is encoded by the CXCL10 gene. The gene is located on human chromosome 4 in a cluster among several other CXC chemokines. CXCL 10 is a small cytokine belonging to the CXC chemokine family. It is secreted by several cell types in response to IFN-gamma. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/ macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.