Free Fatty Acid Assay Kit

Produktnummer ABIN1000307

Produktdetails

Target: Free Fatty Acid (FFA)

Applikation: Biochemical Assay (BCA)

Proben: Serum, Plasma, Urine, Saliva, Milk, Cell Culture Supernatant, Food

Spezifität: 7 μM fatty acid

Produktmerkmale: Sensitive. Use 10 µL samples. Linear detection range: colorimetric assay 7 - 1000 µM, fluorimetric assay 7 - 100 µM fatty acid.
Convenient. Room temperature mix-and-read procedure can be readily automated for high-throughput assay of thousands of samples per day.

Bestandteile: Assay Buffer: 20 mL. Dye Reagent: 120 µL. Enzyme A: 120 µL. Enzyme B: 120 µL. CoSubstrate: 120 µL. Standard: 1 mL 1 mM palmitic acid.

Benötigtes Material: Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader, black flat-bottom uncoated 96-well plates, fluorescence plate reader. For milk and solid samples, 0.45µm PTFE syringe filter and 5% isopropanol, 5% Triton X-100 solution.

Anwendungsinformationen

Applikationshinweise: Assays: free fatty acids in biological samples such as serum, plasma, urine, saliva, milk, cell cultures and in food, agriculture products.
Drug Discovery/Pharmacology: effects of drugs on free fatty acid metabolism.

Ergebnisberechnung:

Subtract blank value (#4) from the standard values and plot the OD or F against standard concentrations.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Referenzen

Jin, Norris, Vaziri: "Dysregulation of hepatic fatty acid metabolism in chronic kidney disease." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 28, Issue 2, pp. 313-20, (2013) (PubMed).

Lu, Zhang, Li, Jin, Huang: "TLR4 antagonist reduces early-stage atherosclerosis in diabetic apolipoprotein E-deficient mice." in: The Journal of endocrinology, Vol. 216, Issue 1, pp. 61-71, (2013) (PubMed).

Hwang, Lee, Huh, Park, Lee, Ho, Ha: "Catalase deficiency accelerates diabetic renal injury through peroxisomal dysfunction." in: Diabetes, Vol. 61, Issue 3, pp. 728-38, (2012) (PubMed).

Jelinek, Castillo, Richardson, Luo, Heidenreich, Garver: "The Niemann-Pick C1 gene is downregulated in livers of C57BL/6J mice by dietary fatty acids, but not dietary cholesterol, through feedback inhibition of the SREBP pathway." in: The Journal of nutrition, Vol. 142, Issue 11, pp. 1935-42, (2012) (PubMed).

Seo, Um, Rico, Kang: "Antihyperlipidemic and body fat-lowering effects of silk proteins with different fibroin/sericin compositions in mice fed with high fat diet." in: Journal of agricultural and food chemistry, Vol. 59, Issue 8, pp. 4192-7, (2011) (PubMed).

Primassin, Tucci, Spiekerkoetter: "Hepatic and muscular effects of different dietary fat content in VLCAD deficient mice." in: Molecular genetics and metabolism, Vol. 104, Issue 4, pp. 546-51, (2011) (PubMed).

Antigendetails

Target: Free Fatty Acid (FFA)

Andere Bezeichnung: Free Fatty Acid (FFA Produkte)

Hintergrund: Quantitative determination of free fatty acid by colorimetric (570nm) or fluorimetric (530/590nm) method.
Procedure: 30 min.

Fatty acids are aliphatic monocarboxylic acids that are ubiquitously found in animal or vegetable fat, oil and wax. Fatty Acids play important roles in cellular synthesis, energy metabolism and are implicated in diverse disorders such as diabetes mellitus, sudden infant death syndrome and Reye Syndrome. This method provides a simple, one-step and high-throughput assay for measuring free fatty acids. In this assay, free fatty acids are enzymatically converted to acyl- CoA and subsequently to H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The optical density at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the free fatty acid concentration in the sample.