Choline Assay Kit

Produktnummer ABIN1000299

Kurzübersicht für Choline Assay Kit (ABIN1000299)

Target: Choline

Applikation: Biochemical Assay (BCA)

Proben: Cell Culture Supernatant, Cell Culture Supernatant, Milk, Plasma, Saliva, Serum, Urine

Produktdetails

Produktmerkmale: Use 20 µL samples. Linear detection range: colorimetric assay 1 to 100 µM, fluorimetric assay 0.2 to 10 µM choline.

Bestandteile: Assay Buffer: 10 mL. Enzyme Mix: 120 µL. Dye Reagent: 120 µL. Standard: 400 µL 2 mM Choline.

Benötigtes Material: Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader, black flat-bottom uncoated 96-well plates, fluorescence plate reader.

Anwendungsinformationen

Applikationshinweise: Assays: choline in biological samples such as serum, plasma, urine, saliva, milk, tissue, and cell culture.
Drug Discovery/Pharmacology: effects of drugs on choline metabolism.

Ergebnisberechnung:

Subtract blank value (#4) from the standard values and plot the OD or F against standard concentrations.
Conversions: 1 mM choline equals 10.4 mg/dL, 0.010% or 104 ppm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Referenzen

Strilakou, Tsakiris, Kalafatakis, Stylianaki, Karkalousos, Koulouris, Mourouzis, Liapi: "Carnitine modulates crucial myocardial adenosine triphosphatases and acetylcholinesterase enzyme activities in choline-deprived rats." in: Canadian journal of physiology and pharmacology, Vol. 92, Issue 1, pp. 78-84, (2014) (PubMed).

Antigendetails

Target: Choline

Hintergrund: Quantitative determination of choline by colorimetric (570nm) or fluorometric (530nm/590nm) methods.
Procedure: 30 min.

Choline and its metabolites play important roles in membrane structure integrity, cellular signaling and cholinergic neurotransmission. Aberrant regulation in choline metabolism has been associated with mental illness such as anxiety. This method provides a simple, direct and high-throughput assay for measuring choline in biological samples. In this assay, free choline is oxidized by choline oxidase to betaine and H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the choline concentration in the sample.