Glucose Assay Kit

Details zu Produkt Nr. ABIN1000263
Antigen
Applikation
Biochemical Assay (BCA)
Optionen
Proben Serum, Plasma, Food, Beverages
Spezifität 0.7 mg/dL (39 μM)
Produktmerkmale Sensitive and accurate. Use as little as 5 µL samples. Linear detection range 0.7 mg/dL (39 µM) to 300 mg/dL (16.6 mM) glucose in 96-well plate.
Simple and convenient. The procedure involves addition of a single working reagent and incubation for 8 min in a boiling water bath.
Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.
Low interference in biological samples.
No pretreatments are needed. Assays can be directly performed on serum and plasma samples.
Bestandteile Reagent: 50 ml. Standard: 1 ml 300 mg/dL.
Benötigtes Material Pipeting devices, centrifuge tubes, boiling water bath, tube holder. Clear bottom 96-well plates (e.g. Corning Costar) and plate reader. Spectrophotometer and Cuvets for measuring OD at 620-650nm.
Protein- /Substanz
Hintergrund Quantitative determination of glucose by chemical colorimetric (630nm) method.
Procedure: 10 min.

Glucose (C6H12O6) is a ubiquitous fuel molecule in biology. It is oxidized through a series of enzyme-catalyzed reactions to form carbon dioxide and water, yielding the universal energy molecule ATP. Due to its importance in metabolism, glucose level is a key diagnostic parameter for many metabolic disorders. Increased glucose levels have been associated with diabetes mellitus, hyperactivity of thyroid, pituitary and adrenal glands. Decreased levels are found in insulin secreting tumors, myxedema, hypopituitarism and hypoadrenalism. Simple, direct and automation-ready procedures for measuring glucose concentrations find wide applications in research and drug discovery. This glucose assay kit is designed to measure glucose directly in serum or plasma without any pretreatment. The improved o-toluidine method utilizes a specific color reaction with glucose. The absorbance at 630nm is directly proportional to glucose concentration in the sample.
Applikations-hinweise Direct Assays: glucose in biological samples (e.g. serum and plasma).
Drug Discovery/Pharmacology: effects of drugs on glucose metabolism.
Food and Beverages: glucose in food, beverages etc.
Protokoll THE REAGENT CONTAINS ACETIC ACID. THIS ASSAY IS PREFERABLY CARRIED OUT IN A CHEMICAL FUME HOOD.

Procedure using 96-well plate:
1. Dilute standard in distilled water. Set up1.5-mL centrifuge tubes. Transfer 5 µL diluted standards and samples to appropriately labeled tubes. Transfer 500 µL Reagent to each tube. Close the tubes tightly and mix. Store diluted standards at -20°C for future use.
2. Place the tubes in a tube holder and heat in a boiling water bath or heat block for 8 min. Cool down in cold water bath for 4 min.
3. Transfer 200 µL in duplicate into a clear bottom 96-well plate. Careful: avoid bubble formation. Read optical density at 620-650nm (peak absorbance at 630nm).

Procedure using cuvette:
1. Dilute standards and transfer 12 µL water blank, Standards and samples to appropriately labeled tubes. Transfer 1200 µL Reagent to each tube. Close the tubes tightly and mix.
2. Place the tubes in a tube holder and heat in a boiling water bath for 8 min. Cool down in cold- water bath for 4 min.
3. Transfer 1000 µL reaction mixture into cuvet. Read optical density at 620-650nm (peak absorbance at 630nm) against blank. Note:
1. if the Sample OD is higher than the Standard OD at 300mg/dL, dilute sample in water and repeat the assay.
2. To determine low glucose concentrations, use 50 µL sample and standards (instead of 5 µL) per 500 µL Reagent.
Ergebnisberechnung

Subtract blank OD (water, #5) from the standard OD values and plot the OD against standard concentrations. Determine the slope using linear regression fitting. Typical serum/plasma glucose values: 70 - 110 mg/dL.
Conversions: 1mg/dL glucose equals 55.5 µM, 0.001% or 10 ppm.

Beschränkungen Nur für Forschungszwecke einsetzbar
Lagerung 4 °C
Bilder des Herstellers
Biochemical Assay (BCA) image for Glucose Assay Kit (ABIN1000263) Glucose Assay Kit
Produkt verwendet in: Hedbacker, Birsoy, Wysocki, Asilmaz, Ahima, Farooqi, Friedman: "Antidiabetic effects of IGFBP2, a leptin-regulated gene." in: Cell metabolism, Vol. 11, Issue 1, pp. 11-22, 2010 (PubMed).

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Stites, Storms, Bauerly, Mah, Harris, Fascetti, Rogers, Tchaparian, Satre, Rucker: "Pyrroloquinoline quinone modulates mitochondrial quantity and function in mice." in: The Journal of nutrition, Vol. 136, Issue 2, pp. 390-6, 2006 (PubMed).

Jatana, Giri, Ansari, Elango, Singh, Singh, Khan: "Inhibition of NF-kappaB activation by 5-lipoxygenase inhibitors protects brain against injury in a rat model of focal cerebral ischemia." in: Journal of neuroinflammation, Vol. 3, pp. 12, 2006 (PubMed).

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