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Maus IgM Isotyp-Kontrolle

Name: Maus IgM Isotyp-Kontrolle
SKU: ABIN964517
Price: 245.28 EUR
Availability: InStock

FACS, ELISA, WB, SDS, FM, FLISA Monoclonal MML IgM unconjugated

SDS-PAGE of Mouse IgM lambda isotype control. (Maus IgM Isotyp-Kontrolle)

SDS

SDS-PAGE of Mouse IgM Lambda (λ) isotype control . (Maus IgM Isotyp-Kontrolle)

SDS

2 Abbildungen

Produktnummer ABIN964517

Hersteller Produkt- Nr.: 010-001-338

Datenblatt (PDF)

Kurzübersicht für Maus IgM Isotyp-Kontrolle (ABIN964517)

Target

IgM

Klonalität

Monoklonal

Applikation

Flow Cytometry (FACS), ELISA, Western Blotting (WB), SDS-PAGE (SDS), Fluorescence Microscopy (FM), FLISA

Klon

MML

Isotyp

IgM

Wirt
46

37

34

5

4

2

2

2

2

2

2

2

2

2

2

1

1
Maus

Hersteller Produkt- Nr.

010-001-338

Hersteller

Rockland

Verwendungszweck

Mouse IgM Lambda (λ) Isotype Control

Kreuzreaktivität (Details)

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgM mu and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, Ig3, IgA. Light chain composition has been confirmed by SDS-PAGE.

Produktmerkmale

Mouse IgM Lambda isotype Control, Mouse IgM Lambda subclass isotype Control

Aufreinigung

Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A.

Sterilität

Sterile filtered
Mouse IgM isotype control (FITC)
FACS, ELISA Monoclonal 11E10 IgM FITC

Mouse IgM isotype control (PE)
FACS, ELISA Monoclonal 11E10 IgM PE

Rat IgM isotype control
FACS, ELISA Monoclonal NIP-M-2 IgM unconjugated

Mouse IgM isotype control
IsoC, FACS, ELISA, WB, IHC (fro), IHC (p), ICC, IP Monoclonal PFR-03 IgM unconjugated

Rabbit IgM isotype control
IsoC, ELISA, IHC Polyclonal IgM unconjugated

Mouse IgM isotype control (APC)
FACS, ELISA Monoclonal 11E10 IgM APC

Rat IgM isotype control (PE)
FACS, ELISA Monoclonal NIP-M-2 IgM PE

Mouse IgM isotype control (Biotin)
FACS, ELISA Monoclonal 11E10 IgM Biotin

Mouse IgM isotype control (SPRD)
FACS, ELISA Monoclonal 11E10 IgM SPRD

Mouse IgM isotype control (PE-Cy7)
FACS, ELISA Monoclonal 11E10 IgM PE-Cy7

Applikationshinweise

Flow Cytometry Dilution: 1:1000-1:5000
Application Note: Mouse IgM lambda isotype control has been tested in SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Western Blot Dilution: User Optimized
FLISA Dilution: User Optimized
ELISA Dilution: User Optimized
IF Microscopy Dilution: User Optimized
Other: User Optimized

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1.0 mg/mL

Buffer

Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Stabilizer: None
Preservative: 0.1 % (w/v) Sodium Azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Haltbarkeit

12 months
Target

IgM

Substanzklasse

Antibody

Hintergrund

Background: Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and μ - IgM, light chain kappa and lambda.