Human IgM Isotyp-Kontrolle
Kurzübersicht für Human IgM Isotyp-Kontrolle (ABIN964504)
Target
Fragment
Applikation
Isotyp
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Wirt
- Human
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Hersteller Produkt- Nr.
- 009-0131
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Hersteller
- Rockland
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Verwendungszweck
- Human IgM (myeloma) Fc5μ Fragment
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Kreuzreaktivität (Details)
- A single precipitin arc was observed against anti-human IgM Fc5μ and anti-human serum when assayed by immuno-electrophoresis at a concentration of 20 mg/ml. No reaction was observed against anti-Trypsin, anti-human IgG F(ab')2, anti-human IgG F(c), anti-human Kappa or anti-human Lambda.
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Produktmerkmale
- Human immunoglobulin M fragment, Human IgM Fc5μ
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Aufreinigung
- Human IgM (myeloma) Fc5μ fragment has been prepared from Human IgM myeloma protein by digestion with trypsin followed by column chromatography.
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Reinheit
- Purity was assessed by SDS-PAGE and HPLC to be greater than 95%.
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Sterilität
- Sterile filtered
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Applikationshinweise
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Immunohistochemistry Dilution: User Optimized
Application Note: Human IgM (myeloma) Fc5μ fragment has been tested in SDS-Page and can be used in Western Blotting and ELISA experiments as a control reagent.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: User Optimized
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1.0 mg/mL
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Buffer
- Optional[Buffer]: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0 Preservative: 0.05 % (w/v) Sodium Azide
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store vial at 4° C prior to opening. Human IgM (myeloma) Fc5μ fragment is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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Haltbarkeit
- 12 months
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- IgM
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Substanzklasse
- Antibody
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Hintergrund
- Background: Human IgM (myeloma) Fc5μ fragment consists of only the μ (mu) chain of the Fc fragment. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. IgM is formed from covalently linking 5 immunoglobulins together. Due to this large size, IgM is typically isolated to the serum.
Target
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