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Human IgM Isotyp-Kontrolle

ELISA, WB, SDS, IHC IgM unconjugated Fc5μ fragment
Rockland
Produktnummer ABIN964504
Hersteller Produkt- Nr.: 009-0131
363,37 €
Zzgl. Versandkosten 20,00 € und MwSt
1 mg
Lieferung nach: Deutschland
Lieferung in 5 bis 7 Werktagen

Kurzübersicht für Human IgM Isotyp-Kontrolle (ABIN964504)

Target

IgM

Fragment

Fc5μ fragment

Applikation

ELISA, Western Blotting (WB), SDS-PAGE (SDS), Immunohistochemistry (IHC)

Isotyp

IgM
  • Wirt

    • 67
    • 56
    • 37
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human

    Hersteller Produkt- Nr.

    009-0131

    Hersteller

    Rockland

    Verwendungszweck

    Human IgM (myeloma) Fc5μ Fragment

    Kreuzreaktivität (Details)

    A single precipitin arc was observed against anti-human IgM Fc5μ and anti-human serum when assayed by immuno-electrophoresis at a concentration of 20 mg/ml.  No reaction was observed against anti-Trypsin, anti-human IgG F(ab')2, anti-human IgG F(c), anti-human Kappa or anti-human Lambda.

    Produktmerkmale

    Human immunoglobulin M fragment, Human IgM Fc5μ

    Aufreinigung

    Human IgM (myeloma) Fc5μ fragment has been prepared from Human IgM myeloma protein by digestion with trypsin followed by column chromatography. 

    Reinheit

    Purity was assessed by SDS-PAGE and HPLC to be greater than 95%. 

    Sterilität

    Sterile filtered
  • Applikationshinweise

    Immunohistochemistry Dilution: User Optimized

    Application Note: Human IgM (myeloma) Fc5μ fragment has been tested in SDS-Page and can be used in Western Blotting and ELISA experiments as a control reagent.

    Western Blot Dilution: User Optimized

    ELISA Dilution: User Optimized

    Other: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1.0 mg/mL

    Buffer

    Optional[Buffer]: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0 Preservative: 0.05 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at 4° C prior to opening.   Human IgM (myeloma) Fc5μ fragment is stable 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.

    Haltbarkeit

    12 months
  • Target

    IgM

    Substanzklasse

    Antibody

    Hintergrund

    Background: Human IgM (myeloma) Fc5μ fragment consists of only the μ (mu) chain of the Fc fragment. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. IgM is formed from covalently linking 5 immunoglobulins together. Due to this large size, IgM is typically isolated to the serum.
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