Kurzübersicht für Ratte IgM isotype control (FITC) (ABIN964494)
Target
Konjugat
Applikation
Isotyp
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Wirt
- Ratte
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Hersteller Produkt- Nr.
- 012-0207
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Hersteller
- Rockland
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Verwendungszweck
- Rat IgM Whole Molecule Fluorescein Conjugated
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Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rat Serum and anti-Rat IgM (μ chain specific). No reaction was observed against anti-Rat IgG F(c). Some light chain cross reactivity will occur with anti-Rat IgG.
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Produktmerkmale
- Rat IgM fluorescein conjugate, Rat IgM FITC conjugated
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Aufreinigung
- This product was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.
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Labeling Ratio
- 20.8
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Mouse IgM isotype control (FITC)
FACS, ELISA Monoclonal 11E10 IgM FITC
Rat IgM isotype controlFACS, ELISA Monoclonal NIP-M-2 IgM unconjugated
Mouse IgM isotype controlIsoC, FACS, ELISA, WB, IHC (fro), IHC (p), ICC, IP Monoclonal PFR-03 IgM unconjugated
Mouse IgM isotype control (PE)FACS, ELISA Monoclonal 11E10 IgM PE
Rabbit IgM isotype controlIsoC, ELISA, IHC Polyclonal IgM unconjugated
Mouse IgM isotype control (Biotin)FACS, ELISA Monoclonal 11E10 IgM Biotin
Rat IgM isotype control (PE)FACS, ELISA Monoclonal NIP-M-2 IgM PE
Mouse IgM isotype control (APC)FACS, ELISA Monoclonal 11E10 IgM APC
Mouse IgM isotype control (SPRD)FACS, ELISA Monoclonal 11E10 IgM SPRD
Mouse IgM isotype control (FITC)IsoC, FACS Monoclonal PFR-03 IgM FITC
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Applikationshinweise
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Application Note: Rat IgM whole molecule Fluorescein is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Other: User Optimized
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 500 μL
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Konzentration
- 0.5 mg/mL
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Buffer
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Buffer: 0.06 M Potassium Phosphate, 0.45 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Haltbarkeit
- 12 months
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- IgM
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Substanzklasse
- Antibody
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Hintergrund
- Background: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. IgM whole molecule is ideal for investigators in Immunology, Microbiology, and Cell Biology.
Target
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