Maus IgG3 Isotyp-Kontrolle
Kurzübersicht für Maus IgG3 Isotyp-Kontrolle (ABIN964483)
Target
Klonalität
Applikation
Klon
Isotyp
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Wirt
- Maus
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Hersteller Produkt- Nr.
- 010-001-336
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Hersteller
- Rockland
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Verwendungszweck
- Mouse IgG3 Kappa (κ) Isotype Control
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Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG3 and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgA, IgM. Light chain composition has been confirmed by SDS-PAGE.
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Produktmerkmale
- Mouse IgG3 isotype control, Mouse IgG3 subclass isotype control, mouse IgG3 kappa subclass control
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Aufreinigung
- Mouse IgG3 Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A.
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Sterilität
- Sterile filtered
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Mouse IgG3 isotype control (PE)
FACS, ELISA Monoclonal B10 IgG3 PE
Mouse IgG3 isotype control (FITC)FACS, ELISA Monoclonal B10 IgG3 FITC
Mouse IgG3 isotype control (Biotin)FACS, ELISA Monoclonal B10 IgG3 Biotin
Mouse IgG3 isotype control (APC)FACS, ELISA Monoclonal B10 IgG3 APC
Mouse IgG3 isotype control (SPRD)FACS, ELISA Monoclonal B10 IgG3 SPRD
Mouse IgG3 isotype controlFACS, ELISA Monoclonal B10 IgG3 unconjugated
Mouse IgG3 isotype controlFACS, IsoC, ELISA, IHC Monoclonal B10 IgG3 unconjugated
Human IgG3 isotype controlIsoC Polyclonal IgG3 unconjugated
Mouse IgG3 isotype controlFACS, IsoC, ELISA, WB, ICC, IP, IHC (fro), IHC (p) Monoclonal PPV-07 IgG3 unconjugated
Mouse IgG3 isotype control (PE)FACS, IsoC Monoclonal PPV-07 IgG3 PE
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Applikationshinweise
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Flow Cytometry Dilution: 1:1000-1:5000
Application Note: Mouse IgG3 kappa isotype control has been tested in SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Western Blot Dilution: User Optimized
FLISA Dilution: User Optimized
ELISA Dilution: User Optimized
IF Microscopy Dilution: User Optimized
Other: User Optimized
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 0.953 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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Haltbarkeit
- 12 months
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- IgG3
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Substanzklasse
- Antibody
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Hintergrund
- Background: Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and μ - IgM, light chain kappa and lambda.
Target
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