Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

Maus IgG3 isotype control (FITC)

FACS, ELISA, WB, FLISA, FM, DB Monoclonal MG3 IgG3 FITC
Rockland
Produktnummer ABIN964480
Hersteller Produkt- Nr.: 010-0243

Kurzübersicht für Maus IgG3 isotype control (FITC) (ABIN964480)

Target

IgG3

Klonalität

  • 38
  • 2
  • 1
Monoklonal

Konjugat

FITC

Applikation

Flow Cytometry (FACS), ELISA, Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Klon

  • 18
  • 6
  • 4
  • 4
  • 2
  • 1
  • 1
  • 1
MG3

Isotyp

IgG3
  • Wirt

    • 56
    • 6
    • 6
    • 1
    • 1
    Maus

    Hersteller Produkt- Nr.

    010-0243

    Hersteller

    Rockland

    Verwendungszweck

    Mouse IgG3 Isotype Control Fluorescein Conjugated

    Kreuzreaktivität (Details)

    Typically less than 1% cross reactivity against other mouse heavy or light chain specific antibodies was detected by ELISA.

    Produktmerkmale

    Fluorescein conjugated Mouse IgG3 isotype control, Mouse IgG3 subclass isotype control FITC conjugation

    Aufreinigung

    This product has been prepared from serum-free tissue culture supernate by protein A chromatography. 

    Labeling Ratio

    3.3
  • Applikationshinweise

    Flow Cytometry Dilution: 1:1000-1:5000

    Application Note: Mouse IgG3 isotype has been tested in dot blot and is designed for immunofluorescence microscopy, flow cytometry, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    Western Blot Dilution: User Optimized

    FLISA Dilution: User Optimized

    ELISA Dilution: User Optimized

    IF Microscopy Dilution: User Optimized

    Other: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Reconstitution Volume: 100 μL

    Konzentration

    1.0 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

    Haltbarkeit

    12 months
  • Target

    IgG3

    Substanzklasse

    Antibody

    Hintergrund

    Background: Isotype controls are important for Flow Cytometry and have no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.
Sie sind hier:
Chat with us!