Maus IgG3 isotype control (FITC)
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- Target Alle IgG3 Produkte
- IgG3
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Wirt
- Maus
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Klonalität
- Monoklonal
- Konjugat
- FITC
- Applikation
- Flow Cytometry (FACS), ELISA, Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)
- Hersteller Produkt- Nr.
- 010-0243
- Hersteller
- Rockland
- Verwendungszweck
- Mouse IgG3 Isotype Control Fluorescein Conjugated
- Kreuzreaktivität (Details)
- Typically less than 1% cross reactivity against other mouse heavy or light chain specific antibodies was detected by ELISA.
- Produktmerkmale
- Fluorescein conjugated Mouse IgG3 isotype control, Mouse IgG3 subclass isotype control FITC conjugation
- Aufreinigung
- This product has been prepared from serum-free tissue culture supernate by protein A chromatography.
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Klon
- MG3
- Isotyp
- IgG3
- Labeling Ratio
- 3.3
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- Applikationshinweise
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Flow Cytometry Dilution: 1:1000-1:5000
Application Note: Mouse IgG3 isotype has been tested in dot blot and is designed for immunofluorescence microscopy, flow cytometry, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Western Blot Dilution: User Optimized
FLISA Dilution: User Optimized
ELISA Dilution: User Optimized
IF Microscopy Dilution: User Optimized
Other: User Optimized
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 100 μL
- Konzentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Haltbarkeit
- 12 months
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- Target
- IgG3
- Abstract
- IgG3 Produkte
- Substanzklasse
- Antibody
- Hintergrund
- Background: Isotype controls are important for Flow Cytometry and have no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.
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