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Maus IgG3 isotype control (FITC)

FACS, ELISA, WB, FLISA, FM, DB Monoclonal MG3 IgG3 FITC
Rockland
Produktnummer ABIN964480
Hersteller Produkt- Nr.: 010-0243
  • Target Alle IgG3 Produkte
    IgG3
    Wirt
    • 49
    • 6
    • 6
    • 1
    • 1
    Maus
    Klonalität
    • 31
    • 2
    • 1
    Monoklonal
    Konjugat
    FITC
    Applikation
    Flow Cytometry (FACS), ELISA, Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)
    Hersteller Produkt- Nr.
    010-0243
    Hersteller
    Rockland
    Verwendungszweck
    Mouse IgG3 Isotype Control Fluorescein Conjugated
    Kreuzreaktivität (Details)
    Typically less than 1% cross reactivity against other mouse heavy or light chain specific antibodies was detected by ELISA.
    Produktmerkmale
    Fluorescein conjugated Mouse IgG3 isotype control, Mouse IgG3 subclass isotype control FITC conjugation
    Aufreinigung
    This product has been prepared from serum-free tissue culture supernate by protein A chromatography. 
    Klon
    • 18
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    MG3
    Isotyp
    IgG3
    Labeling Ratio
    3.3
  • Applikationshinweise

    Flow Cytometry Dilution: 1:1000-1:5000

    Application Note: Mouse IgG3 isotype has been tested in dot blot and is designed for immunofluorescence microscopy, flow cytometry, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    Western Blot Dilution: User Optimized

    FLISA Dilution: User Optimized

    ELISA Dilution: User Optimized

    IF Microscopy Dilution: User Optimized

    Other: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Reconstitution Volume: 100 μL

    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Haltbarkeit
    12 months
  • Target
    IgG3
    Abstract
    IgG3 Produkte
    Substanzklasse
    Antibody
    Hintergrund
    Background: Isotype controls are important for Flow Cytometry and have no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.
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