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Maus IgG3 Isotyp-Kontrolle

Name: Maus IgG3 Isotyp-Kontrolle
SKU: ABIN964479
Price: 271.79 EUR
Availability: InStock

FACS, ELISA, WB, FLISA, FM, SDS Monoclonal MG3 IgG3 unconjugated

SDS-PAGE of Mouse IgG3. (Maus IgG3 Isotyp-Kontrolle)

SDS

1 image

Produktnummer ABIN964479

Hersteller Produkt- Nr.: 010-0143

Kurzübersicht für Maus IgG3 Isotyp-Kontrolle (ABIN964479)

Target

IgG3

Klonalität

Monoklonal

Applikation

Flow Cytometry (FACS), ELISA, Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), SDS-PAGE (SDS)

Klon

MG3

Isotyp

IgG3

Wirt
50

6

6

1

1
Maus

Hersteller Produkt- Nr.

010-0143

Hersteller

Rockland

Verwendungszweck

Mouse IgG3 Isotype Control

Kreuzreaktivität (Details)

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG3 and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgA, IgM. Confirmed by SDS-PAGE.

Produktmerkmale

MOUSE IgG3 subclass isotype control, MOUSE IgG3 isotype control

Aufreinigung

Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A.

Sterilität

Sterile filtered
Mouse IgG3 isotype control (PE)
FACS, ELISA Monoclonal B10 IgG3 PE

Mouse IgG3 isotype control (FITC)
FACS, ELISA Monoclonal B10 IgG3 FITC

Mouse IgG3 isotype control (APC)
FACS, ELISA Monoclonal B10 IgG3 APC

Mouse IgG3 isotype control (SPRD)
FACS, ELISA Monoclonal B10 IgG3 SPRD

Mouse IgG3 isotype control (Biotin)
FACS, ELISA Monoclonal B10 IgG3 Biotin

Mouse IgG3 isotype control (PE-Cy7)
FACS, ELISA Monoclonal B10 IgG3 PE-Cy7

Mouse IgG3 isotype control
FACS, ELISA Monoclonal B10 IgG3 unconjugated

Mouse IgG3 isotype control
FACS, ELISA, IsoC, IHC Monoclonal B10 IgG3 unconjugated

Human IgG3 isotype control
IsoC Polyclonal IgG3 unconjugated

Mouse IgG3 isotype control
FACS, ELISA, IsoC, WB, IHC (fro), IHC (p), ICC, IP Monoclonal PPV-07 IgG3 unconjugated

Applikationshinweise

Flow Cytometry Dilution: 1:1000-1:5000
Application Note: Mouse IgG3 isotype control has been tested by SDS-Page and ELISA and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Western Blot Dilution: User Optimized
FLISA Dilution: User Optimized
ELISA Dilution: User Optimized
IF Microscopy Dilution: User Optimized
Other: User Optimized

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1.0 mg/mL

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Store vial at 4° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit

12 months
Target

IgG3

Substanzklasse

Antibody

Hintergrund

Background: Isotype controls are important for Flow Cytometry and have no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.