Maus IgG2b isotype control (Biotin)
Kurzübersicht für Maus IgG2b isotype control (Biotin) (ABIN964476)
Target
Klonalität
Konjugat
Applikation
Klon
Isotyp
-
-
Wirt
- Maus
-
Hersteller Produkt- Nr.
- 010-0642
-
Hersteller
- Rockland
-
Verwendungszweck
- Mouse IgG2b Isotype Control Biotin Conjugated
-
Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2b and anti-Mouse serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgM, and IgA. Biotin Conjugated Mouse IgG2b was proven by Dot Blot.
-
Produktmerkmale
- biotin conjugation Mouse IgG2b isotype control, MOUSE IgG2b subclass isotype control biotin conjugated
-
Aufreinigung
- Mouse IgG2b isotype control has been prepared from in vitro cell culture by selective precipitation.
-
-
Mouse IgG2b isotype control (Biotin)
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b Biotin
Mouse IgG2b isotype controlFACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b unconjugated
Mouse IgG2b isotype control (APC)FACS, IsoC Monoclonal MPC-11 IgG2b APC
Mouse IgG2b isotype control (FITC)FACS, IsoC Monoclonal MPC-11 IgG2b FITC
Mouse IgG2b isotype control (PE)FACS, IsoC Monoclonal MPC-11 IgG2b PE
Mouse IgG2b isotype control (PerCP)FACS, IsoC Monoclonal MPC-11 IgG2b PerCP
Mouse IgG2b isotype controlFACS, ELISA Monoclonal A-1 IgG2b unconjugated
Mouse IgG2b isotype controlFACS, IsoC, ELISA, WB Monoclonal A-1 IgG2b unconjugated
Mouse IgG2b isotype control (Cy3)FACS, ELISA Monoclonal A-1 IgG2b Cy3
Mouse IgG2b isotype control (PE)FACS, ELISA Monoclonal A-1 IgG2b PE
-
-
Applikationshinweise
-
Flow Cytometry Dilution: 1:1000-1:5000
Application Note: Mouse IgG2b isotype control can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
FLISA Dilution: User Optimized
IF Microscopy Dilution: 1:1000-1:5000
Other: User Optimized
-
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Format
- Lyophilized
-
Rekonstitution
-
Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 100 μL
-
Konzentration
- 0.5 mg/mL
-
Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Konservierungsmittel
- Sodium azide
-
Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Lagerung
- 4 °C,-20 °C
-
Informationen zur Lagerung
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
Haltbarkeit
- 12 months
-
-
- IgG2b
-
Substanzklasse
- Antibody
-
Hintergrund
- Background: Isotype controls are important for Flow Cytometry and have no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.
Target
-
