Maus IgG2a isotype control (FITC)
Kurzübersicht für Maus IgG2a isotype control (FITC) (ABIN964463)
Target
Klonalität
Konjugat
Applikation
Klon
Isotyp
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Wirt
- Maus
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Hersteller Produkt- Nr.
- 010-0241
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Hersteller
- Rockland
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Verwendungszweck
- Mouse IgG2a Isotype Control Fluorescein Conjugated
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Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2a and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Fluorescein Conjugated Mouse IgG2a was proven by Dot Blot.
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Produktmerkmale
- Fluorescein conjugated MOUSE IgG2a subclass isotype control, MOUSE IgG2a isotype control FITC conjugation
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Aufreinigung
- This product has been prepared from normal mouse serum by immunoaffinity chromatography using antibodies coupled to agarose beads. Extensive cross-adsorbtion was performed to remove any unwanted subclasses.
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Labeling Ratio
- 4.99
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Mouse IgG2a isotype control (APC)
FACS, IsoC Monoclonal MOPC-173 IgG2a kappa APC
Mouse IgG2a isotype control (PerCP)FACS, IsoC Monoclonal MOPC-173 IgG2a kappa PerCP
Mouse IgG2a isotype controlFACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a kappa unconjugated
Mouse IgG2a isotype controlFACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), Func, IHC (p) Monoclonal MOPC-173 IgG2a kappa unconjugated
Rat IgG2a isotype control (FITC)FACS, IsoC Monoclonal RTG2A1-1 IgG2a FITC
Mouse IgG2a isotype control (FITC)FACS, IsoC Monoclonal MOPC-173 IgG2a kappa FITC
Mouse IgG2a isotype control (Biotin)FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a kappa Biotin
Mouse IgG2a isotype control (PE)FACS, IsoC Monoclonal MOPC-173 IgG2a kappa PE
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Applikationshinweise
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Flow Cytometry Dilution: 1:1000-1:5000
Application Note: Mouse IgG2a isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, Immunofluorescence, and ELISA experiments where determination of sample isotype is important.
Western Blot Dilution: User Optimized
FLISA Dilution: User Optimized
ELISA Dilution: User Optimized
IF Microscopy Dilution: User Optimized
Other: User Optimized
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 100 μL
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Konzentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Haltbarkeit
- 12 months
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- IgG2a
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Substanzklasse
- Antibody
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Hintergrund
- Background: Isotype controls are important for Flow Cytometry and have no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.
Target
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