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Maus IgG1 Isotyp-Kontrolle

Rockland 010-001-331 FACS, ELISA, WB, FM, FLISA, SDS Monoclonal MG1L IgG1 unconjugated
Rockland
Produktnummer ABIN964460
Hersteller Produkt- Nr.: 010-001-331
  • Target Alle IgG1 Produkte
    IgG1
    Wirt
    • 223
    • 47
    • 28
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    Maus
    Klonalität
    • 177
    • 11
    • 6
    Monoklonal
    Applikation
    Flow Cytometry (FACS), ELISA, Western Blotting (WB), Fluorescence Microscopy (FM), FLISA, SDS-PAGE (SDS)
    Hersteller Produkt- Nr.
    010-001-331
    Hersteller
    Rockland
    Verwendungszweck
    Mouse IgG1 Lambda (λ) Isotype Control
    Kreuzreaktivität (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum.  Isotyping assay resulted non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgA, IgM. Light chain composition has been confirmed by SDS-PAGE.
    Produktmerkmale
    Mouse immunoglobulin gamma lambda, Mouse IgG1 isotype control, Mouse IgG1 Lambda
    Aufreinigung
    Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. 
    Sterilität
    Sterile filtered
    Klon
    • 45
    • 29
    • 18
    • 12
    • 9
    • 7
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    MG1L
    Isotyp
    IgG1
  • Applikationshinweise

    Flow Cytometry Dilution: 1:1000-1:5000

    Application Note: Mouse IgG1 lambda isotype control has been tested in SDS-Page and ELISA and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

    Western Blot Dilution: User Optimized

    FLISA Dilution: User Optimized

    ELISA Dilution: User Optimized

    IF Microscopy Dilution: User Optimized

    Other: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to opening.   Mouse IgG1 Lambda isotype control is stable 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.
    Haltbarkeit
    12 months
  • Klaile, Müller, Schäfer, Clauder, Feer, Heyl, Stock, Klassert, Zipfel, Singer, Slevogt: "Binding of Candida albicans to Human CEACAM1 and CEACAM6 Modulates the Inflammatory Response of Intestinal Epithelial Cells." in: mBio, Vol. 8, Issue 2, (2017) (PubMed).

  • Target
    IgG1
    Abstract
    IgG1 Produkte
    Substanzklasse
    Antibody
    Hintergrund
    Background: Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and μ - IgM, light chain kappa and lambda. IgG1 is the most abundant of the four IgG subclasses. This isotype control possesses lambda light chains.
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