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Maus IgG1 Isotyp-Kontrolle

Name: Maus IgG1 Isotyp-Kontrolle
SKU: ABIN964460
Price: 245.28 EUR
Availability: InStock

FACS, ELISA, WB, FM, FLISA, SDS Monoclonal MG1L IgG1 unconjugated

SDS-PAGE of Mouse IgG1 Lambda Isotype Control. (Maus IgG1 Isotyp-Kontrolle)

SDS

1 image

(1 reference)

Produktnummer ABIN964460

Hersteller Produkt- Nr.: 010-001-331

Kurzübersicht für Maus IgG1 Isotyp-Kontrolle (ABIN964460)

Target

IgG1

Klonalität

Monoklonal

Applikation

Flow Cytometry (FACS), ELISA, Western Blotting (WB), Fluorescence Microscopy (FM), FLISA, SDS-PAGE (SDS)

Klon

MG1L

Isotyp

IgG1

Wirt
221

59

28

6

4

2

2

1

1

1
Maus

Hersteller Produkt- Nr.

010-001-331

Hersteller

Rockland

Verwendungszweck

Mouse IgG1 Lambda (λ) Isotype Control

Kreuzreaktivität (Details)

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgA, IgM. Light chain composition has been confirmed by SDS-PAGE.

Produktmerkmale

Mouse immunoglobulin gamma lambda, Mouse IgG1 isotype control, Mouse IgG1 Lambda

Aufreinigung

Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A.

Sterilität

Sterile filtered
Mouse IgG1 isotype control (Biotin)
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-21 IgG1 kappa Biotin

Mouse IgG1 isotype control (FITC)
FACS, ELISA Monoclonal 15H6 IgG1 FITC

Mouse IgG1 isotype control (PE)
FACS, ELISA Monoclonal 15H6 IgG1 PE

Mouse IgG1 isotype control
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p), Func Monoclonal MOPC-21 IgG1 kappa unconjugated

Mouse IgG1 isotype control
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-21 IgG1 kappa unconjugated

Mouse IgG1 isotype control (SPRD)
FACS, ELISA Monoclonal 15H6 IgG1 SPRD

Mouse IgG1 isotype control (APC)
FACS, ELISA Monoclonal 15H6 IgG1 APC

Mouse IgG1 isotype control (Biotin)
FACS, ELISA Monoclonal 15H6 IgG1 Biotin

Mouse IgG1 isotype control (PE-Cy7)
FACS, ELISA Monoclonal 15H6 IgG1 PE-Cy7

Mouse IgG1 isotype control (APC-Cy7)
FACS, ELISA Monoclonal 15H6 IgG1 APC-Cy7

Applikationshinweise

Flow Cytometry Dilution: 1:1000-1:5000
Application Note: Mouse IgG1 lambda isotype control has been tested in SDS-Page and ELISA and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Western Blot Dilution: User Optimized
FLISA Dilution: User Optimized
ELISA Dilution: User Optimized
IF Microscopy Dilution: User Optimized
Other: User Optimized

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1.0 mg/mL

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Store vial at 4° C prior to opening. Mouse IgG1 Lambda isotype control is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Haltbarkeit

12 months
Klaile, Müller, Schäfer, Clauder, Feer, Heyl, Stock, Klassert, Zipfel, Singer, Slevogt: "Binding of Candida albicans to Human CEACAM1 and CEACAM6 Modulates the Inflammatory Response of Intestinal Epithelial Cells." in: mBio, Vol. 8, Issue 2, (2017) (PubMed).
Target

IgG1

Substanzklasse

Antibody

Hintergrund

Background: Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and μ - IgM, light chain kappa and lambda. IgG1 is the most abundant of the four IgG subclasses. This isotype control possesses lambda light chains.