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Ratte IgG Isotyp-Kontrolle

Rockland 012-0103 ELISA, WB, SDS, IHC IgG unconjugated Fc fragment
Rockland
Produktnummer ABIN964381
Hersteller Produkt- Nr.: 012-0103
  • Target Alle IgG Produkte
    IgG
    Fragment
    Fc fragment
    Wirt
    • 138
    • 127
    • 119
    • 84
    • 81
    • 81
    • 55
    • 52
    • 46
    • 42
    • 38
    • 33
    • 33
    • 32
    • 25
    • 18
    • 10
    • 8
    • 8
    • 7
    • 4
    • 2
    Ratte
    Applikation
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Immunohistochemistry (IHC)
    Hersteller Produkt- Nr.
    012-0103
    Hersteller
    Rockland
    Verwendungszweck
    Rat IgG Fc Fragment
    Kreuzreaktivität (Details)
    Rat IgG F(c) fragment was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum, anti-Rat IgG and anti-Rat IgG F(c).  No reaction was observed against anti-Rat IgG F(ab')2 or anti-Papain.
    Produktmerkmale
    Rat Immunoglobulin G F(c) fragment, IgG Fc
    Aufreinigung
    Rat IgG F(c) fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. 
    Sterilität
    Sterile filtered
    Isotyp
    IgG
  • Applikationshinweise

    Immunohistochemistry Dilution: User Optimized

    Application Note: Rat IgG F(c) Fragment has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

    Western Blot Dilution: User Optimized

    ELISA Dilution: User Optimized

    Other: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.0 mg/mL
    Buffer
    Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to opening. Stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
    Haltbarkeit
    12 months
  • Target
    IgG
    Abstract
    IgG Produkte
    Substanzklasse
    Antibody
    Hintergrund
    Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The F(c) fragment binds with very high affinity to the Fc receptor proteins on phagocytic leukocytes. When digested from the whole antibody molecule, the F(c) fragment no longer posses the epitope recognition site.
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