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Maus IgG Isotyp-Kontrolle

Name: Maus IgG Isotyp-Kontrolle
SKU: ABIN964336
Price: 255.22 EUR
Availability: InStock

ELISA, WB, SDS, IHC IgG unconjugated Fab fragment

SDS-PAGE of Mouse IgG Fab Fragment . (Maus IgG Isotyp-Kontrolle)

SDS

Coommassie stained SDS-PAGE of 20 µl of Mouse Derived NIH 3T3 Whole Cell Lysate (Ready-to-Use) separated in a 4-20% gradient gel under non-reducing conditions (lane 1). (Maus IgG Isotyp-Kontrolle)

2 Abbildungen

Produktnummer ABIN964336

Hersteller Produkt- Nr.: 010-0105

Datenblatt (PDF)

Kurzübersicht für Maus IgG Isotyp-Kontrolle (ABIN964336)

Target

IgG

Fragment

Fab fragment

Applikation

ELISA, Western Blotting (WB), SDS-PAGE (SDS), Immunohistochemistry (IHC)

Isotyp

IgG

Wirt
137

127

123

84

82

81

55

52

46

42

38

33

33

32

25

18

10

8

8

7

3

2
Maus

Hersteller Produkt- Nr.

010-0105

Hersteller

Rockland

Verwendungszweck

Mouse IgG Fab Fragment

Kreuzreaktivität (Details)

Mouse IgG Fab fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG, anti-Mouse IgG F(ab')2 and anti-Mouse Serum. No reaction was observed against anti-Mouse IgG F(c) or anti- Papain.

Produktmerkmale

Mouse Immunoglobulin Fab, F(ab), Fragment antigen-binding

Aufreinigung

Mouse IgG Fab fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above.

Sterilität

Sterile filtered
Hamster IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated

Rabbit IgG isotype control
FACS, ELISA, WB, IF (cc), IF (p), IHC (fro), IHC (p) Polyclonal IgG unconjugated

Rabbit IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated

Mouse IgG isotype control
FACS, ELISA, WB, IF (cc), IF (p), IHC (fro), IHC (p) Polyclonal IgG unconjugated

Mouse IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated

Rabbit IgG isotype control (PE)
FACS, ELISA Polyclonal IgG PE

Goat IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated

Rabbit IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated

Goat IgG isotype control
IsoC, ELISA, IHC Polyclonal IgG unconjugated

Mouse IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated

Applikationshinweise

Immunohistochemistry Dilution: User Optimized
Application Note: Mouse IgG Fab Fragment has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: User Optimized

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

2.0 mg/mL

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Haltbarkeit

12 months
Target

IgG

Substanzklasse

Antibody

Hintergrund

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.