Maus IgG Isotyp-Kontrolle
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- Target Alle IgG Produkte
- IgG
- Fragment
- Fab fragment
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Wirt
- Maus
- Applikation
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Immunohistochemistry (IHC)
- Hersteller Produkt- Nr.
- 010-0105
- Hersteller
- Rockland
- Verwendungszweck
- Mouse IgG Fab Fragment
- Kreuzreaktivität (Details)
- Mouse IgG Fab fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG, anti-Mouse IgG F(ab')2 and anti-Mouse Serum. No reaction was observed against anti-Mouse IgG F(c) or anti- Papain.
- Produktmerkmale
- Mouse Immunoglobulin Fab, F(ab), Fragment antigen-binding
- Aufreinigung
- Mouse IgG Fab fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above.
- Sterilität
- Sterile filtered
- Isotyp
- IgG
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Rabbit IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated
Mouse IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype control (PE)FACS, ELISA Polyclonal IgG PE
Goat IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Goat IgG isotype controlIsoC, ELISA, IHC Polyclonal IgG unconjugated
Mouse IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype controlIsoC, FACS, ELISA, IHC Polyclonal IgG unconjugated
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- Applikationshinweise
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Immunohistochemistry Dilution: User Optimized
Application Note: Mouse IgG Fab Fragment has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: User Optimized
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 2.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- Haltbarkeit
- 12 months
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- Target
- IgG
- Abstract
- IgG Produkte
- Substanzklasse
- Antibody
- Hintergrund
- Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.
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