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Maus IgG Isotyp-Kontrolle

Name: Maus IgG Isotyp-Kontrolle
SKU: ABIN964335
Price: 245.28 EUR
Availability: InStock

ELISA, WB, SDS, IHC IgG unconjugated F(ab')2 fragment

SDS-PAGE of Mouse IgG F(ab')2 Fragment . (Maus IgG Isotyp-Kontrolle)

SDS

1 image

Produktnummer ABIN964335

Hersteller Produkt- Nr.: 010-0104

Kurzübersicht für Maus IgG Isotyp-Kontrolle (ABIN964335)

Target

IgG

Fragment

F(ab')2 fragment

Applikation

ELISA, Western Blotting (WB), SDS-PAGE (SDS), Immunohistochemistry (IHC)

Isotyp

IgG

Wirt
137

127

123

84

82

80

64

55

46

42

38

33

33

32

25

18

16

10

8

7

3

2
Maus

Hersteller Produkt- Nr.

010-0104

Hersteller

Rockland

Verwendungszweck

Mouse IgG F(ab')2 Fragment

Kreuzreaktivität (Details)

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, anti-Mouse IgG and anti-Mouse IgG F(ab')2. No reaction was observed against anti-Mouse IgG F(c) or anti-Pepsin.

Produktmerkmale

Mouse immunoglobulin Fab, F(ab'), F(ab')2, Fragment antigen-binding

Aufreinigung

Mouse IgG F(ab')2 fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above.
Hamster IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated

Rabbit IgG isotype control
FACS, ELISA, WB, IF (cc), IF (p), IHC (fro), IHC (p) Polyclonal IgG unconjugated

Rabbit IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated

Mouse IgG isotype control
FACS, ELISA, WB, IF (cc), IF (p), IHC (fro), IHC (p) Polyclonal IgG unconjugated

Mouse IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated

Rabbit IgG isotype control (PE)
FACS, ELISA Polyclonal IgG PE

Goat IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated

Rabbit IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated

Goat IgG isotype control
IsoC, ELISA, IHC Polyclonal IgG unconjugated

Mouse IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated

Applikationshinweise

Immunohistochemistry Dilution: User Optimized
Application Note: Mouse IgG F(ab')2 fragment has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: User Optimized

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Lyophilized

Rekonstitution

Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL

Konzentration

2.0 mg/mL

Buffer

Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Mouse IgG F(ab')2 fragment is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit

12 months
Target

IgG

Substanzklasse

Antibody

Hintergrund

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.