Aktivieren Sie Javascript in Ihrem Browser, um unsere Webseite optimal zu nutzen.

Human IgG isotype control (Biotin)

Name: Human IgG isotype control (Biotin)
Brand: Rockland
SKU: ABIN964324
Availability: InStock

ELISA, WB, IHC, DB IgG Biotin Fc fragment

Dot Blot of Human IgG F(c) Fragment Biotin Conjugated. (Human IgG isotype control (Biotin))

1 image

Produktnummer ABIN964324

Hersteller Produkt- Nr.: 009-0603

402,65 €

Zzgl. Versandkosten 20,00 € und MwSt

1 mg

Lieferung nach: Deutschland

Lieferung in 5 bis 7 Werktagen

Kurzübersicht für Human IgG isotype control (Biotin) (ABIN964324)

Target

IgG

Fragment

Fc fragment

Konjugat

Biotin

Applikation

ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB)

Isotyp

IgG

Wirt
140

139

138

94

92

80

60

55

46

42

38

35

34

32

25

18

15

14

11

8

3

2
Human

Hersteller Produkt- Nr.

009-0603

Hersteller

Rockland

Verwendungszweck

Human IgG Fc Fragment Biotin Conjugated

Kreuzreaktivität (Details)

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human IgG, anti-Human IgG F(c) and anti-Human Serum. No reaction was observed against anti-Human IgG F(ab')2 or anti-Papain.

Produktmerkmale

Human Immunoglobulin G F(c) fragment, IgG Fc, Vitamin H, Coenzyme R

Aufreinigung

Human IgG F(c) fragment Biotin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above.
Rabbit IgG isotype control
IsoC Polyclonal IgG unconjugated

Hamster IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated

Rabbit IgG isotype control
FACS, ELISA, WB, IF (cc), IF (p), IHC (fro), IHC (p) Polyclonal IgG unconjugated

Rabbit IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated

Rabbit IgG isotype control
FACS, IHC, IP Polyclonal IgG unconjugated

Rabbit IgG isotype control (Atto 550)
FACS, IHC Polyclonal IgG Atto 550

Mouse IgG isotype control
FACS, ELISA, WB, IF (cc), IF (p), IHC (fro), IHC (p) Polyclonal IgG unconjugated

Mouse IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated

Goat IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated

Rabbit IgG isotype control (PE)
FACS, ELISA Polyclonal IgG PE

Applikationshinweise

Immunohistochemistry Dilution: 1:500
Application Note: Human IgG F(c) fragment Biotin conjugated has been tested in dot blot and can be utilized as a control reagent in both Western Blotting and ELISA format experiments.
Western Blot Dilution: 1:1000
ELISA Dilution: 1:5000 - 1:50,000
Other: User Optimized

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Lyophilized

Rekonstitution

Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL

Konzentration

1.0 mg/mL

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Human IgG F(c) fragment Biotin conjugated is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit

12 months
Target

IgG

Substanzklasse

Antibody

Hintergrund

Background: Human IgG Fc purified protein is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of Human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.