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Human IgG Isotyp-Kontrolle

Name: Human IgG Isotyp-Kontrolle
Brand: Rockland
SKU: ABIN964313
Availability: InStock

ELISA, WB, SDS IgG unconjugated F(ab')2 fragment

SDS-Page of Human IgG F(ab’)2 Fragment. (Human IgG Isotyp-Kontrolle)

SDS

1 image

Produktnummer ABIN964313

Hersteller Produkt- Nr.: 009-0104

292,17 €

Zzgl. Versandkosten 20,00 € und MwSt

2 mg

Lieferung nach: Deutschland

Lieferung in 5 bis 8 Werktagen

Kurzübersicht für Human IgG Isotyp-Kontrolle (ABIN964313)

Target

IgG

Fragment

F(ab')2 fragment

Applikation

ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Isotyp

IgG

Wirt
140

139

138

94

92

80

60

55

46

42

38

35

34

32

25

18

15

14

11

8

3

2
Human

Hersteller Produkt- Nr.

009-0104

Hersteller

Rockland

Verwendungszweck

Human IgG F(ab')2 Fragment

Kreuzreaktivität (Details)

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum, anti-Human IgG and anti-Human IgG F(ab')2. No reaction was observed against anti-Human IgG F(c) or anti-Pepsin.

Produktmerkmale

Human Immunoglobulin G F(ab')2 Fragment, IgG Fab2

Aufreinigung

Human IgG F(ab')2 was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above.

Sterilität

Sterile filtered
Rabbit IgG isotype control
IsoC Polyclonal IgG unconjugated

Hamster IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated

Rabbit IgG isotype control
FACS, ELISA, WB, IF (cc), IF (p), IHC (fro), IHC (p) Polyclonal IgG unconjugated

Rabbit IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated

Rabbit IgG isotype control
FACS, IHC, IP Polyclonal IgG unconjugated

Rabbit IgG isotype control (Atto 550)
FACS, IHC Polyclonal IgG Atto 550

Mouse IgG isotype control
FACS, ELISA, WB, IF (cc), IF (p), IHC (fro), IHC (p) Polyclonal IgG unconjugated

Mouse IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated

Goat IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated

Rabbit IgG isotype control (PE)
FACS, ELISA Polyclonal IgG PE

Applikationshinweise

Application Note: Human IgG F(ab')2 Fragment has been tested in SDS-Page and ELISA and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.
Western Blot Dilution: 1:1000
ELISA Dilution: 1:5000 - 1:50,000
Other: User Optimized

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

2.0 mg/mL

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Haltbarkeit

12 months
Target

IgG

Substanzklasse

Antibody

Hintergrund

Background: Human IgG F(ab')2 purified protein is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . Human IgG F(ab')2 Molecules lack the Fc portion of Human IgG and therefore receptors that bind Human IgG Fc will not bind Human IgG F(ab')2 Molecules.