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Ziege IgG isotype control (Rhodamine)

Rockland 005-0003 SDS, DB IgG Rhodamine Fc fragment
Rockland
Produktnummer ABIN964243
Hersteller Produkt- Nr.: 005-0003
  • Target Alle IgG Produkte
    IgG
    Fragment
    Fc fragment
    Wirt
    • 137
    • 127
    • 119
    • 84
    • 82
    • 81
    • 55
    • 52
    • 46
    • 42
    • 38
    • 33
    • 33
    • 32
    • 25
    • 18
    • 10
    • 8
    • 8
    • 7
    • 4
    • 2
    Ziege
    Konjugat
    Rhodamine
    Applikation
    SDS-PAGE (SDS), Dot Blot (DB)
    Hersteller Produkt- Nr.
    005-0003
    Hersteller
    Rockland
    Verwendungszweck
    Goat IgG Fc Fragment Rhodamine Conjugated
    Kreuzreaktivität (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat IgG, anti-Goat IgG F(c) and anti-Goat Serum.  No reaction was observed against anti-Goat IgG F(ab')2 or anti-Papain.
    Produktmerkmale
    Goat IgG F(c) fragment Rhodamine conjugated, Goat IgG F(c) fragment TRITC conjugated
    Aufreinigung
    This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. 
    Isotyp
    IgG
    Labeling Ratio
    4.9
  • Applikationshinweise

    Application Note: Goat IgG F(c) fragment Fluorescein conjugated has been tested in dot blot and SDS-Page and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, and utilizing various commercial platforms.

    Other: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Reconstitution Volume: 1.0 mL

    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Haltbarkeit
    12 months
  • Target
    IgG
    Abstract
    IgG Produkte
    Substanzklasse
    Antibody
    Hintergrund
    Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This antibody possesses the F(c) region, recognized by high-affinity Fc receptor proteins.
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