Huhn IgG isotype control (Biotin)
Kurzübersicht für Huhn IgG isotype control (Biotin) (ABIN964221)
Target
Fragment
Konjugat
Applikation
Isotyp
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Wirt
- Huhn
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Hersteller Produkt- Nr.
- 003-0605
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Hersteller
- Rockland
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Verwendungszweck
- Chicken IgG Fab Fragment Biotin Conjugated
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Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Chicken IgG, anti-Chicken IgG F(ab')2 and anti-Chicken Serum. No reaction was observed against anti-Chicken IgG F(c) or anti-Papain.
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Produktmerkmale
- Chicken IgG Fab fragment Biotin conjugation, Chicken IgG Fab BAC conjugate, Chicken IgY Fab fragment Biotin conjugation, Chicken IgY Fab BAC conjugate
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Aufreinigung
- This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above.
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Applikationshinweise
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Application Note: Chicken IgG F(ab) Biotin Conjugated has been tested by SDS-PAGE and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Other: User Optimized
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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Konzentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Haltbarkeit
- 12 months
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- IgG
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Substanzklasse
- Antibody
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Hintergrund
- Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.
Target
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