Cow IgG isotype control (HRP)
Kurzübersicht für Cow IgG isotype control (HRP) (ABIN964181)
Target
Fragment
Konjugat
Applikation
Isotyp
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Wirt
- Cow
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Hersteller Produkt- Nr.
- 001-0305
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Hersteller
- Rockland
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Verwendungszweck
- Bovine IgG Fab Fragment Peroxidase Conjugated
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Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Bovine IgG, anti-Bovine IgG F(ab')2 and anti-Bovine Serum. No reaction was observed against anti-Bovine IgG F(c) or anti-Papain.
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Produktmerkmale
- Bovine IgG Fab peroxidase conjugation, Bovine IgG Fab HRP conjugation, peroxidase conjugated Bovine IgG Fab, HRP conjugated Bovine IgG Fab fragment
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Aufreinigung
- This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above.
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Applikationshinweise
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Application Note: Bovine IgG F(ab) fragment peroxidase conjugation can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Other: User Optimized
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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Konzentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
Konservierungsmittel
- Gentamicin sulfate
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Vorsichtsmaßnahmen
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Haltbarkeit
- 12 months
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- IgG
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Substanzklasse
- Antibody
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Hintergrund
- Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.
Target
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