Cow IgG Isotyp-Kontrolle
Kurzübersicht für Cow IgG Isotyp-Kontrolle (ABIN964174)
Target
Fragment
Applikation
Isotyp
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Wirt
- Cow
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Hersteller Produkt- Nr.
- 001-0105
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Hersteller
- Rockland
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Verwendungszweck
- Bovine IgG Fab Fragment
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Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine Serum, anti-Bovine IgG and anti-Bovine IgG F(ab')2. No reaction was observed against anti-Bovine IgG F(c) or anti- Papain.
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Produktmerkmale
- Bovine IgG Fab fragment
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Aufreinigung
- This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above.
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Applikationshinweise
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Application Note: Bovine IgG F(ab) has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Other: User Optimized
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 2.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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Haltbarkeit
- 12 months
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- IgG
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Substanzklasse
- Antibody
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Hintergrund
- Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.
Target
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