Hamster IgG isotype control (HRP)

Details zu Produkt Nr. ABIN930151, Anbieter: Anmelden zum Anzeigen
Isotyp
Wirt
Hamster
186
178
177
142
134
117
86
67
67
66
66
60
53
51
38
33
22
16
15
11
7
4
3
Konjugat
HRP
167
155
152
106
106
50
42
41
24
22
13
12
12
11
9
7
6
6
6
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
Applikation
Isotype Control (IsoC)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Isotyp IgG
Fragment F(ab')2 fragment
Produktmerkmale Purified Hamster IgG (Fab'2) HRP conjugate
Alternative Names: Hamster Immunoglobulin G (Fab'2) (HRP)
Physical state: Clear, colorless liquid
Reinigung Human IgG protein (HRP) was purified by delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and dialysis.
Hintergrund Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. The fragment antigen-binding (Fab fragment) is a region on an antibody that binds to antigens. It is composed of one constant and one variable domain of each of the heavy and the light chain. These domains shape the paratope - the antigen-binding site - at the amino terminal end of the monomer. The two variable domains bind the epitope on their specific antigens. Treatment with pepsin produces an F(ab)'2 fragment.
Synonyms: Hamster Immunoglobulin G (Fab'2) (HRP).
Applikations-hinweise Optimal working dilutions should be determined experimentally by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Konzentration Lot specific
Buffer Lyophilized from 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, 10 mg/mL BSA, and 0.01 % gentamicin sulfate. DO NOT add NaN3. Immunoglobulin and protease free.
Konservierungs-mittel Gentamicin sulfate
Vorsichtsmaßnahmen This product contains Gentamicin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handhabung Avoid repeated freeze/thaw cycles.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Lagerung RT/-20 °C
Informationen zur Lagerung Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
Produkt verwendet in: Morris, Brown, Kong: "Naturally-existing CD4(+)CD25(+)Foxp3(+) regulatory T cells are required for tolerance to experimental autoimmune thyroiditis induced by either exogenous or endogenous autoantigen." in: Journal of autoimmunity, Vol. 33, Issue 1, pp. 68-76, 2009 (PubMed).