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Ratte IgG2a isotype control (FITC)

FACS, IsoC Monoclonal RTG2A1-1 IgG2a FITC

Flow cytometry surface nonspecific staining pattern of human peripheral whole blood stained using Rat IgG2a Isotype control (RTG2A1-1) FITC antibody (concentration in sample 9 μg/mL). (Ratte IgG2a isotype control (FITC))

Separation of lymphocytes nonspecific stained using Rat IgG2a Isotype control (RTG2A1-1) FITC antibody (concentration in sample 9 μg/mL)from unstained lymphocytes in flow cytometry analysis. (Ratte IgG2a isotype control (FITC))

2 Abbildungen

Produktnummer ABIN7505834

Datenblatt (PDF)

Kurzübersicht für Ratte IgG2a isotype control (FITC) (ABIN7505834)

Target

IgG2a

Klonalität

Monoklonal

Konjugat

FITC

Applikation

Flow Cytometry (FACS), Isotype Control (IsoC)

Klon

RTG2A1-1

Isotyp

IgG2a

Wirt
169

79

3

1

1
Ratte

Verwendungszweck

Rat IgG2a Isotype Control FITC

Spezifität

This rat IgG2a kappa monoclonal antibody (clone RTG2A1-1, also known as G2A-1-1 or KLH/G2A-1-1) detects specifically the hemocyanin of keyhole limpet and can be used as a negative control antibody in other species.

Aufreinigung

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Mouse IgG2a isotype control (APC)
FACS, IsoC Monoclonal MOPC-173 IgG2a kappa APC

Mouse IgG2a isotype control (PerCP)
FACS, IsoC Monoclonal MOPC-173 IgG2a kappa PerCP

Mouse IgG2a isotype control
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a kappa unconjugated

Mouse IgG2a isotype control
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), Func, IHC (p) Monoclonal MOPC-173 IgG2a kappa unconjugated

Mouse IgG2a isotype control (FITC)
FACS, IsoC Monoclonal MOPC-173 IgG2a kappa FITC

Mouse IgG2a isotype control (Biotin)
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a kappa Biotin

Mouse IgG2a isotype control (PE)
FACS, IsoC Monoclonal MOPC-173 IgG2a kappa PE

Rat IgG2a isotype control (Biotin)
FACS IgG2a Biotin

Rat IgG2a isotype control
FACS, ELISA IgG2a unconjugated

Mouse IgG2a isotype control (FITC)
FACS, ELISA Monoclonal HOPC-1 IgG2a FITC

Applikationshinweise

Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C

Informationen zur Lagerung

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Target

IgG2a

Substanzklasse

Antibody

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