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Human IgM Isotyp-Kontrolle

ELISA, WB IgM unconjugated Fab fragment
Produktnummer ABIN5633258
  • Target Alle IgM Produkte
    IgM
    Fragment
    Fab fragment
    Wirt
    • 61
    • 37
    • 36
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human
    Applikation
    ELISA, Western Blotting (WB)
    Kreuzreaktivität (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum. No reaction was observed against anti-Human IgG F(c). Some light chain cross reactivity will occur with anti-Human IgG.
    Aufreinigung
    HUMAN IgM (myeloma) Fab μ fragment was prepared from serum by a multi-step process which includes delipidation, selective precipitation, tandem molecular sieve chromatography and trypsin digestion followed by extensive dialysis against the buffer stated above.
    Sterilität
    Sterile filtered
    Isotyp
    IgM
  • Applikationshinweise

    Application Note: Monovalent Fab fragments of affinity-purified, secondary antibodies are offered to cover (block) the surface of immunoglobulins for double labeling primary antibodies from the same host species, or to block endogenous immunoglobulins in tissue sections or on cell surfaces. They can be used for these purposes because each Fab fragment has only a single antigen binding site. HUMAN IgM (myeloma) Fab μ fragment can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Specific conditions should be optimized by the user.

    Western Blot Dilution: User Optimized

    ELISA Dilution: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
    Haltbarkeit
    12 months
  • Target
    IgM
    Abstract
    IgM Produkte
    Synonyme
    IgM constant region Isotype Control, IGM Isotype Control
    Substanzklasse
    Antibody
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