Schaf IgG Isotyp-Kontrolle
Kurzübersicht für Schaf IgG Isotyp-Kontrolle (ABIN5633250)
Target
Fragment
Applikation
Isotyp
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Wirt
- Schaf
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Hersteller Produkt- Nr.
- 013-0104-0002
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Hersteller
- Rockland
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Verwendungszweck
- Sheep IgG F(ab')2 Fragment
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Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep IgG, anti-Sheep IgG F(ab')2 and anti-Sheep Serum. No reaction was observed against anti-Sheep IgG F(c) or anti-Pepsin.
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Produktmerkmale
- Sheep IgG F(ab')2 Fragment
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Aufreinigung
- This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above.
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Sterilität
- Sterile filtered
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Hamster IgG isotype control
ELISA, WB, IHC, SDS IgG unconjugated
Rabbit IgG isotype controlELISA, WB, IHC, SDS IgG unconjugated
Rabbit IgG isotype controlFACS, IHC, IP Polyclonal IgG unconjugated
Rabbit IgG isotype control (Atto 550)FACS, IHC Polyclonal IgG Atto 550
Goat IgG isotype controlIsoC, ELISA, IHC Polyclonal IgG unconjugated
Rabbit IgG isotype controlIsoC, FACS, ELISA, IHC Polyclonal IgG unconjugated
Rabbit IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
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Applikationshinweise
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Application Note: Sheep IgG F(ab')2 Fragment has been tested in SDS-Page and are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.
Other: User Optimized
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 2.1 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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Haltbarkeit
- 12 months
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- IgG
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Substanzklasse
- Antibody
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Hintergrund
- Background: Sheep IgG F(ab')2 Fragment is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . Sheep IgG F(ab')2 Molecules lack the Fc portion of Sheep IgG and therefore receptors that bind Sheep IgG Fc will not bind Sheep IgG F(ab')2 Molecules. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.
Target
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