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Ratte IgG Isotyp-Kontrolle

Rockland 012-0102-0005 FACS, ELISA, WB, SDS IgG unconjugated
Rockland
Produktnummer ABIN5633246
Hersteller Produkt- Nr.: 012-0102-0005
  • Target Alle IgG Produkte
    IgG
    Wirt
    • 138
    • 127
    • 119
    • 84
    • 81
    • 81
    • 55
    • 52
    • 46
    • 42
    • 38
    • 33
    • 33
    • 32
    • 25
    • 18
    • 10
    • 8
    • 8
    • 7
    • 4
    • 2
    Ratte
    Applikation
    Flow Cytometry (FACS), ELISA, Western Blotting (WB), SDS-PAGE (SDS)
    Hersteller Produkt- Nr.
    012-0102-0005
    Hersteller
    Rockland
    Verwendungszweck
    Rat IgG Whole Molecule
    Kreuzreaktivität (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat Serum.
    Produktmerkmale
    Rat Immunoglobulin G, Rat IgG whole molecule, Rat IgG purified protein
    Aufreinigung
    Rat IgG was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    Isotyp
    IgG
  • Applikationshinweise

    Flow Cytometry Dilution: User Optimized

    Application Note: Rat IgG whole molecule has been tested by SDS-Page and Western Blot and is suitable for use as antigen or ligand in immunochemical reactions, as a control or standard in assays, for conjugation and most other immunological methods requiring highly purified immunoglobulins.

    Western Blot Dilution: User Optimized

    ELISA Dilution: User Optimized

    Other: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Reconstitution Volume: 500 μL

    Konzentration
    10.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Haltbarkeit
    12 months
  • Target
    IgG
    Abstract
    IgG Produkte
    Substanzklasse
    Antibody
    Hintergrund
    Background: Rat IgG purified protein (Immunoglobulin G) are antibody molecules. Rat IgG is composed of four peptide chains - two heavy chains (gamma) and two light chains. Rat IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. Rat IgG typically constitutes 75 % of serum immunoglobulins. Rat IgG molecules are synthesized and secreted by plasma B cells.
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