Cow IgG Isotyp-Kontrolle
Kurzübersicht für Cow IgG Isotyp-Kontrolle (ABIN5633217)
Target
Fragment
Applikation
Isotyp
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Wirt
- Cow
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Hersteller Produkt- Nr.
- 001-0104-0002
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Hersteller
- Rockland
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Verwendungszweck
- Bovine IgG F(ab')2 Fragment
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Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine IgG, anti-Bovine IgG F(ab')2 and anti-Bovine Serum. No reaction was observed against anti-Bovine IgG F(c) or anti-Pepsin.
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Produktmerkmale
- Bovine IgG F(ab')2 fragment, Bovine IgG Fab2 fragment
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Aufreinigung
- This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above.
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Sterilität
- Sterile filtered
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Applikationshinweise
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Application Note: Bovine IgG F(ab')2 has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Other: User Optimized
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 2.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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Haltbarkeit
- 12 months
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- IgG
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Substanzklasse
- Antibody
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Hintergrund
- Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.
Target
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