Ratte IgG2b Isotyp-Kontrolle
Kurzübersicht für Ratte IgG2b Isotyp-Kontrolle (ABIN5608829)
Target
Klonalität
Applikation
Klon
Isotyp
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Wirt
- Ratte
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Hersteller Produkt- Nr.
- 012-001-p04
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Hersteller
- Rockland
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Verwendungszweck
- Rat IgG2c Kappa (κ) Isotype Control
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Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum. Isotyping assay resulted non-reactive with antisera to Rat IgG1a, IgG1b, IgG3, IgM, and IgA. Allophycocyanin Conjugated Rat IgG2c confirmed by Dot Blot.
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Produktmerkmale
- Rat Isotype Control, Rat IgG2c isotype, Rat IgG2c kappa isotype, Rat IgG2 Kappa
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Aufreinigung
- RAT IgG2c Kappa isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G.
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Sterilität
- Sterile filtered
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Mouse IgG2b isotype control (Biotin)
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b Biotin
Mouse IgG2b isotype controlFACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b unconjugated
Mouse IgG2b isotype control (APC)FACS, IsoC Monoclonal MPC-11 IgG2b APC
Mouse IgG2b isotype control (FITC)FACS, IsoC Monoclonal MPC-11 IgG2b FITC
Mouse IgG2b isotype control (PE)FACS, IsoC Monoclonal MPC-11 IgG2b PE
Mouse IgG2b isotype control (PerCP)FACS, IsoC Monoclonal MPC-11 IgG2b PerCP
Mouse IgG2b isotype controlFACS, ELISA Monoclonal A-1 IgG2b unconjugated
Mouse IgG2b isotype controlFACS, IsoC, ELISA, WB Monoclonal A-1 IgG2b unconjugated
Mouse IgG2b isotype control (Cy3)FACS, ELISA Monoclonal A-1 IgG2b Cy3
Mouse IgG2b isotype control (PE)FACS, ELISA Monoclonal A-1 IgG2b PE
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Applikationshinweise
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Flow Cytometry Dilution: 1:1000 - 1:5000
Application Note: RAT IgG2c Kappa isotype control has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.
Western Blot Dilution: User Optimized
FLISA Dilution: User Optimized
ELISA Dilution: User Optimized
IF Microscopy Dilution: User Optimized
Other: User Optimized
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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Haltbarkeit
- 12 months
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- IgG2b
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Andere Bezeichnung
- IgG2c
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Substanzklasse
- Antibody
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Hintergrund
- Background: RAT IgG2c Kappa isotype control is used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In Rat there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 2c and μ - IgM, light chain kappa and lambda.
Target
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