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Rattete IgG2a isotype control (FITC)

FACS, ELISA, FLISA, FM Monoclonal IgG2a FITC
Produktnummer ABIN5608809
  • Target Alle IgG2a Produkte
    IgG2a
    Reaktivität
    Ratte
    Wirt
    • 184
    • 73
    • 3
    • 1
    Ratte
    Klonalität
    • 171
    • 2
    • 1
    Monoklonal
    Konjugat
    FITC
    Applikation
    Flow Cytometry (FACS), ELISA, FLISA, Fluorescence Microscopy (FM)
    Aufreinigung
    RAT IgG2a isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to rat IgG1, IgG2b, IgG3 , IgM , and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum. Light and heavy chain composition has been confirmed.
    Isotyp
    IgG2a
    Labeling Ratio
    2-8
  • Applikationshinweise

    Flow Cytometry Dilution: 1:1000-1:5000

    Application Note: RAT IgG2a isotype control can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.

    FLISA Dilution: User Optimized

    ELISA Dilution: 1:2000-1:20,000

    IF Microscopy Dilution: User Optimized

    Kommentare

    IgG2a isotype control is used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In Rat there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain α - IgA, γ - IgG 1, 2a, 2b, 2c and μ - IgM, light chain κ and λ.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.0 mg/mL
    Buffer

    Stabilizer: None

    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
    Haltbarkeit
    12 months
  • Target
    IgG2a
    Abstract
    IgG2a Produkte
    Synonyme
    gamma-2a immunoglobulin heavy chain Isotype Control, IgG-2a Isotype Control
    Substanzklasse
    Antibody
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