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Llama IgG3 Isotyp-Kontrolle

FACS, ELISA, WB IgG3 unconjugated
Rockland
Produktnummer ABIN5608800
Hersteller Produkt- Nr.: 025-0143
235,70 €
Zzgl. Versandkosten 20,00 €, Trockeneispauschale 20,00 € sowie MwSt
0.5 mg
Lieferung nach: Deutschland
Lieferung in 5 bis 7 Werktagen

Kurzübersicht für Llama IgG3 Isotyp-Kontrolle (ABIN5608800)

Target

IgG3

Applikation

Flow Cytometry (FACS), ELISA, Western Blotting (WB)

Isotyp

IgG3
  • Reaktivität

    Llama

    Wirt

    • 71
    • 15
    • 6
    • 5
    • 1
    • 1
    Llama

    Hersteller Produkt- Nr.

    025-0143

    Hersteller

    Rockland

    Aufreinigung

    Llama IgG3 isotype control has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG3 shows a band of ~100 kDa whereas the reduced form exhibits ~43 kDa. No bands corresponding to llama IgG1 or IgG2 are observed.
  • Applikationshinweise

    Flow Cytometry Dilution: User Optimized

    Application Note: Llama IgG3 isotype control can be utilized as a control or standard reagent in Flow Cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

    Western Blot Dilution: User Optimized

    ELISA Dilution: User Optimized

    Kommentare

    Comparative studies of old world camelids (Camelus bactrianus and Camelus dromedarius) and new world camelids (Lama pacos, Lama glama and Lama vicugna) have shown that heavy-chain-only immunoglobulins represent between 35 % - 70 % of total IgG in the sera of all species. Such antibodies are homodimers of heavy chains that lack the CH1 domain of conventional antibodies and therefore do not interact with light chains, exhibiting a lower molecular weight ~100 kDa. In llama and other species of camelids, these heavy-chain-only immunoglobulins belong to the IgG2 and IgG3 subclasses of gamma chain antibodies.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1.0 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Target

    IgG3

    Substanzklasse

    Antibody
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