Ratte IgG1 isotype control (Biotin)
Kurzübersicht für Ratte IgG1 isotype control (Biotin) (ABIN5608797)
Target
Klonalität
Konjugat
Applikation
Klon
Isotyp
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Wirt
- Ratte
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Hersteller Produkt- Nr.
- 012-006-040
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Hersteller
- Rockland
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Verwendungszweck
- Rat IgG1 Isotype Control Biotin Conjugated
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Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rat IgG, and anti-Rat serum. Isotyping assay resulted non-reactive with antisera to Rat IgG2a, IgG2b, IgG3, IgM, and IgA. Biotin Conjugated Rat IgG1 confirmed by Dot Blot.
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Produktmerkmale
- Rat Isotype Control, Rat IgG1 Biotin isotype, RAT IgG1 subclass isotype control
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Aufreinigung
- RAT IgG1 isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G.
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Mouse IgG1 isotype control (Biotin)
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-21 IgG1 Biotin
Mouse IgG1 isotype control (Cy5)FACS, ELISA Monoclonal 15H6 IgG1 Cy5
Mouse IgG1 isotype control (PE)FACS, ELISA Monoclonal 15H6 IgG1 PE
Mouse IgG1 isotype control (APC)FACS, ELISA Monoclonal 15H6 IgG1 APC
Mouse IgG1 isotype control (SPRD)FACS, ELISA Monoclonal 15H6 IgG1 SPRD
Mouse IgG1 isotype control (APC-Cy7)FACS, ELISA Monoclonal 15H6 IgG1 APC-Cy7
Mouse IgG1 isotype control (APC-Cy5.5)FACS, ELISA Monoclonal 15H6 IgG1 APC-Cy5.5
Mouse IgG1 isotype control (PE-Cy7)FACS, ELISA Monoclonal 15H6 IgG1 PE-Cy7
Mouse IgG1 isotype control (FITC)FACS, ELISA Monoclonal 15H6 IgG1 FITC
Mouse IgG1 isotype control (FITC)FACS, IsoC, DFA, IFA VI-AP IgG1 FITC
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Applikationshinweise
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Flow Cytometry Dilution: User Optimized
Application Note: RAT IgG1 isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: User Optimized
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 50μL
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Konzentration
- 1 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Haltbarkeit
- 12 months
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- IgG1
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Substanzklasse
- Antibody
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Hintergrund
- Background: RAT IgG1 isotype control is used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In Rat there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 2c and μ - IgM, light chain kappa and lambda.
Target
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