Ratte IgG Isotyp-Kontrolle
-
- Target Alle IgG Produkte
- IgG
-
Wirt
- Ratte
- Applikation
- Isotype Control (IsoC), ELISA, Western Blotting (WB), Dot Blot (DB), Immunodiffusion (ID), Haemagglutination (H), Binding Studies (Bind)
- Produktmerkmale
- Identity and purity is confirmed by immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) using antisera to rat total serum proteins, to rat IgG, IgA, IgM, transferrin and albumin
- Aufreinigung
- Chromatographically purified rat IgG
- Reinheit
- > = 96 % as determined by agar-gel electrophoresis. Tested in a concentration of 70.0 mg/mL at the level of sensitivity of the applied immunoprecipitating techniques, this product contains only traces of IgA, IgM, transferrin and albumin.
- Isotyp
- IgG
-
-
- Applikationshinweise
- Purified rat IgG may be used as a reference antigen, calibrator, coating protein and blocking agent in a variety of immunoassays including immunodiffusion, immunoelec-trophoresis, ELISA, Western blotting, dot-immunobinding assays (DIBA), haemagglutination, and cell-binding assays.
- Kommentare
-
Lyophilized, stable white powder.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Lyophilized
- Rekonstitution
- After opening the vial, the lyophilized content is reconstituted by adding 1 mL of sterile distilled water, mixed gently by inversion until complete dissolution is obtained. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium.
- Konservierungsmittel
- Without preservative
- Handhabung
- Working dilutions may be prepared by adding the required amount of phosphate buffered saline (PBS), should not be refrozen and preferably used within 24 hours. Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- This product is shipped lyophilized at ambient temperature. After arrival store at +4 °C. Prolonged storage may be at -20 °C. Reconstituted solution may be stored frozen.
-
- Target
- IgG
- Abstract
- IgG Produkte
- Substanzklasse
- Antibody
-