Maus IgG Isotyp-Kontrolle
Kurzübersicht für Maus IgG Isotyp-Kontrolle (ABIN458536)
Target
Applikation
Isotyp
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Wirt
- Maus
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Produktmerkmale
- Identity and purity is confirmed by immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) using antisera to mouse total serum proteins and specific antisera.
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Aufreinigung
- Chromatographically purified mouse IgG
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Reinheit
- > = 98 % as determined by agar-gel electrophoresis. Tested in a concentration of 23 mg/mL at the level of sensitivity of the applied immunoprecipitating techniques, this product contains a trace of transferrin, but no reaction has been observed with antisera against IgA, IgM and C3c.
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Applikationshinweise
- Purified mouse IgG may be used as a reference antigen, calibrator, coating protein and blocking agent in a variety of immunoassays including immunodiffusion, immunoelec- trophoresis, ELISA, Western blotting, dot-immunobinding assays (DIBA), haemagglutination, and cell-binding assays.
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Kommentare
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Lyophilized, stable white powder.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- After opening the vial, the lyophilized content is reconstituted by adding 1 mL of sterile distilled water, mixed gently by inversion until complete dissolution is obtained. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium.
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Konservierungsmittel
- Without preservative
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Handhabung
- Working dilutions may be prepared by adding the required amount of phosphate buffered saline (PBS), should not be refrozen and preferably used within 24 hours. Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product.
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Lagerung
- 4 °C/-20 °C
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Informationen zur Lagerung
- This product is shipped lyophilized at ambient temperature. After arrival store at +4 °C. Prolonged storage may be at -20 °C. Reconstituted solution may be stored frozen.
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- IgG
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Substanzklasse
- Antibody
Target
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