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Maus IgG2a isotype control (PerCP)

FACS, IsoC Monoclonal MOPC-173 IgG2a kappa PerCP
Produktnummer ABIN2749155

Kurzübersicht für Maus IgG2a isotype control (PerCP) (ABIN2749155)

Target

IgG2a

Klonalität

  • 159
  • 2
  • 1
Monoklonal

Konjugat

PerCP

Applikation

Flow Cytometry (FACS), Isotype Control (IsoC)

Klon

  • 28
  • 17
  • 15
  • 12
  • 10
  • 6
  • 6
  • 5
  • 5
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
MOPC-173

Isotyp

IgG2a kappa
  • Wirt

    • 168
    • 80
    • 3
    • 1
    • 1
    Maus

    Verwendungszweck

    Mouse IgG2a Isotype Control PerCP

    Spezifität

    This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.

    Keine Kreuzreaktivität

    Human, Maus, Ratte

    Aufreinigung

    Purified antibody is conjugated with activated Peridinin-Chlorophyll Protein (PerCP) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

    Immunogen

    The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
  • Applikationshinweise

    Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    0.1 mg/mL

    Buffer

    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C

    Informationen zur Lagerung

    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Khoddami, Cairns: "Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP." in: Nature protocols, Vol. 9, Issue 2, pp. 337-61, (2014) (PubMed).

    Gupta, Gylling, Alonso, Sugimori, Ianakiev, Xiong, Arnaout: "The beta-tail domain (betaTD) regulates physiologic ligand binding to integrin CD11b/CD18." in: Blood, Vol. 109, Issue 8, pp. 3513-20, (2007) (PubMed).

    Fougereau, Bourgois, de Preval, Rocca-Serra, Schiff: "The complete sequence of the murine monoclonal immunoglobulin MOPC 173 (IgG2a): genetic implications." in: Annales d'immunologie, Vol. 127, Issue 5, pp. 607-31, (1977) (PubMed).

    Baumal, Scharff: "Immunoglobulin biosynthesis by the MOPC 173 mouse myeloma tumor and a variant spleen clone." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 116, Issue 1, pp. 65-74, (1976) (PubMed).

  • Target

    IgG2a

    Andere Bezeichnung

    IgG2a kappa

    Substanzklasse

    Antibody
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