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Maus IgG2a isotype control (PerCP)

Name: Maus IgG2a isotype control (PerCP)
Brand: antibodies-online
SKU: ABIN2749155
Price: 356.73 EUR
Availability: InStock

FACS, IsoC Monoclonal MOPC-173 IgG2a kappa PerCP

Flow cytometry surface nonspecific staining pattern of human peripheral whole blood stained using mouse IgG2a Isotype control (MOPC-173) PerCP antibody (concentration in sample 5 μg/mL). (Maus IgG2a isotype control (PerCP))

FACS

Example of nonspecific mouse IgG2a (MOPC-173) PerCP signal on human peripheral blood, surface staining, 9 μg/mL. (Maus IgG2a isotype control (PerCP))

FACS

2 Abbildungen

(4 Referenzen)

Produktnummer ABIN2749155

Datenblatt (PDF)

Kurzübersicht für Maus IgG2a isotype control (PerCP) (ABIN2749155)

Target

IgG2a

Klonalität

Monoklonal

Konjugat

PerCP

Applikation

Flow Cytometry (FACS), Isotype Control (IsoC)

Klon

MOPC-173

Isotyp

IgG2a kappa

Wirt
168

80

3

1

1
Maus

Verwendungszweck

Mouse IgG2a Isotype Control PerCP

Spezifität

This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Keine Kreuzreaktivität

Human, Maus, Ratte

Aufreinigung

Purified antibody is conjugated with activated Peridinin-Chlorophyll Protein (PerCP) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen

The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
Mouse IgG2a isotype control (APC)
FACS, IsoC Monoclonal MOPC-173 IgG2a kappa APC

Mouse IgG2a isotype control
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a kappa unconjugated

Mouse IgG2a isotype control
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), Func, IHC (p) Monoclonal MOPC-173 IgG2a kappa unconjugated

Rat IgG2a isotype control (FITC)
FACS, IsoC Monoclonal RTG2A1-1 IgG2a FITC

Mouse IgG2a isotype control (FITC)
FACS, IsoC Monoclonal MOPC-173 IgG2a kappa FITC

Mouse IgG2a isotype control (Biotin)
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a kappa Biotin

Mouse IgG2a isotype control (PE)
FACS, IsoC Monoclonal MOPC-173 IgG2a kappa PE

Rat IgG2a isotype control (Biotin)
FACS IgG2a Biotin

Rat IgG2a isotype control
FACS, ELISA IgG2a unconjugated

Mouse IgG2a isotype control (FITC)
FACS, ELISA Monoclonal HOPC-1 IgG2a FITC

Applikationshinweise

Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

0.1 mg/mL

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C

Informationen zur Lagerung

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Khoddami, Cairns: "Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP." in: Nature protocols, Vol. 9, Issue 2, pp. 337-61, (2014) (PubMed).

Gupta, Gylling, Alonso, Sugimori, Ianakiev, Xiong, Arnaout: "The beta-tail domain (betaTD) regulates physiologic ligand binding to integrin CD11b/CD18." in: Blood, Vol. 109, Issue 8, pp. 3513-20, (2007) (PubMed).

Fougereau, Bourgois, de Preval, Rocca-Serra, Schiff: "The complete sequence of the murine monoclonal immunoglobulin MOPC 173 (IgG2a): genetic implications." in: Annales d'immunologie, Vol. 127, Issue 5, pp. 607-31, (1977) (PubMed).

Baumal, Scharff: "Immunoglobulin biosynthesis by the MOPC 173 mouse myeloma tumor and a variant spleen clone." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 116, Issue 1, pp. 65-74, (1976) (PubMed).
Target

IgG2a

Andere Bezeichnung

IgG2a kappa

Substanzklasse

Antibody