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Ratte IgG2a isotype control (Biotin)

FACS, IsoC IgG2a Biotin
Produktnummer ABIN2688784
  • Target Alle IgG2a Produkte
    IgG2a
    Wirt
    • 184
    • 73
    • 3
    • 1
    Ratte
    Konjugat
    Biotin
    Applikation
    Flow Cytometry (FACS), Isotype Control (IsoC)
    Produktmerkmale
    The R35-95 hybridoma was generated by hybridization of Y3 myeloma cells with spleen cells from LOU rats immunized with mouse immunoglobulins. The R35-95 hybridoma produces rat IgG2a, κ immunoglobulin that has no measurable reactivity with mouse immunoglobulins. The R35-95 immunoglobulin was selected as an isotype control following screening for low background binding on a variety of mouse and human tissues. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA)
    Aufreinigung
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Mouse Pooled Immunoglobulin
    Isotyp
    IgG2a
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Probenmenge
    20 μL
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Informationen zur Lagerung
    The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.
  • Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).

  • Target
    IgG2a
    Abstract
    IgG2a Produkte
    Synonyme
    gamma-2a immunoglobulin heavy chain Isotype Control, IgG-2a Isotype Control
    Substanzklasse
    Antibody
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